464 



EXPERIMENTAL PHARMACOLOGY 



When all adjustments are made take a normal record 

 and then pour a few drops of ammonium hydroxide solu- 

 tion on the towel so that the animal inhales the vapors. 

 How does this affect the respiration, bladder, spleen vol- 

 ume and heart rate? How do you explain the results? 

 What nerves are involved in the reactions! 



Remove the ammonia and insert a tracheal cannula. 



Fig. 355. Tracing showing the action of yanadium on the volume of an excised, perfused 



segment of the small intestine of a dog. 



Take a normal record and then inject one cubic centimeter 

 of the ammonium chloride solution. Is this action identical 

 in its origin with that produced by the inhalation of am- 

 monia fumes? Inject a larger dose of ammonium chloride 

 to get satisfactory records if the first ones were not good 

 enough (but save the vitality of the animal as much as pos- 

 sible). Empty out the ammonium chloride solution and 

 place adrenaline (1:10,000) in the burette. 



Allow the animal to recover as fully as possible. Take 



