CHEMICAL 309 



TABLE I. TOTAL NITROGEN IN LUPINUS LUTEUS: RESULTS OBTAINED 

 BY STOKLASA. (PERCENTAGE ON DRY BASIS). 



Period. Roots. Nodules. 



Blooming 1.64 5.22 



Fruiting 1.84 2.61 



Maturity 1.42 1.73 



"Stoklasa also determined protein, amids, and asparagin in 

 lupine nodules. The protein was obtained by the Stutzer method, 

 the amids by the Kjeldahl method, and the asparagin by calcula- 

 tion from the ammonia obtained by distillation with magnesium oxid. 

 Table II shows his results. 



TABLE II. NITROGEN COMPOUNDS IN LUPINE NODULES: RESULTS 

 OBTAINED BY STOKLASA. (PERCENTAGE ON DRY BASIS). 



Period. Protein. Amids. Asparagin. 



Blossoming 3.99 0.35 0.34 



Maturity 1.54 0.15 Trace 



The presence of asparagin in the nodule is important, as it is 

 thought to be intimately related with the formation of protein. 



"In 1901 Wassilieff studied the nitrogen compounds in white 

 lupine (Lupinus alba) seeds and seedlings. He found that the seeds 

 contained 7.68 per cent, of total nitrogen; and that of this, 6.89 per 

 cent, was in the form of protein and 0.53 per cent, was precipitated by 

 phosphotungstic acid, leaving a difference of 0.26 per cent., aspara- 

 gin. The occurrence of asparagin in large amounts in the seed- 

 lings is shown by the data given in Table III. 



TABLE III. NITROGEN COMPOUNDS IN FOURTEEN-DAY-OLD GREEN 

 SEEDLINGS OF WHITE LUPINES: RESULTS OBTAINED BY 

 WASSILIEFF (EXPRESSED IN PERCENTAGE ON DRY BASIS). 



Total 

 Protein. nitrogen. 



4.11 6.57 



2.44 7.83 



1.56 6.77 



1.87 5.40 



Wassilieff also demonstrated the presence of leucin and tyrosin 

 in the cotyledons of one-week-old seedlings of white lupines. These 

 and other amino-acids would be expected to be present when the 

 protein of the seed is breaking down for the nutrition of the seedling. 



"Knisely analyzed the leaves, pods, stems, roots, and nodules 

 of lupine plants for total nitrogen at three distinct periods of develop- 

 ment. His results show better than the others presented where the 

 nitrogen accumulates as the plant matures. 



1 Phosphotungstic acid. 



