CHEMICAL STUDIES OF THE CONTENT OF MICROBIAL CELLS 189 



in Chlorella vulgar is (alga) to 10.73 in B. proteus (Proteus mdgaris). 

 In the protozoon, Noctiluca miharis, there was present 7. 74 per cent, of 

 nitrogen as determined by Emmerling. * Molds and yeasts appear to 

 lie between the alga named and many of the bacteria as indicated by the 

 work of Marschall and Nageli.f 



The compounds of nitrogen which have been determined are quite 

 numerous although it must be allowed that the analyses have not always 

 been satisfactory. RuppelJ claims to have determined nucleic acid, 

 nucleoprotamin, nucleoproteid, albuminoids (keratin etc.) in dried 

 Bact. tuberculosis. Nishimura|| found nuclein bodies as xanthin, 

 guanin, adenin in a water bacillus. Vaughan and his associates have 

 been able to demonstrate the presence of various amino acids. The 

 work of Emmerling* also contributes much which aids in our under- 

 standing of definite substances in the protoplasm of protozoa. 



* Emmerling, O., Biochem. Zeitschr., 1909, gives this analysis of Noctiluca miharis: In 100 

 grams of ash free substance there was 7.74 grams of nitrogen (Taken from S. von Prowazek: 

 Physiologic der Einzelligen.) 



Lysin 0.212 with o . 040 grams nitrogen 



Arginin i .6492 with o .432 grams nitrogen 



Histidin 3.4762 with 0.938 grams nitrogen 



Tyrosin 0.5271 with 0.041 grams nitrogen 



Glycocoll 15 .9000 with 2 .956 grams nitrogen 



Alanin 2 . 4000 with 0.378 grams nitrogen 



Leucin o . 4200 with o . 044 grams nitrogen 



Prolin 4 .6000 with o .556 grams nitrogen 



Asparagin acid, o . 1700 with o .020 grams nitrogen 



Total 5 405 grams nitrogen. 



t Marschall, Arch. f. Hyg., 28, 19, estimates the protein in Aspergillus at 30.4 per cent., in 

 Penicillium at 40.2 per cent., and Mucor at 43.4 per cent, (based upon dry weight). In Arch, 

 f. Hyg. 28, 1917, 17, the per cent, of protein in molds is placed at 38.0. 



Nageli and Loew., Jour. Prakt. Chem. N. F., 17, determined 47.0 per cent, of protein in 

 yeasts. 



% Ruppel. Zeit. f. Physiol. Chemie, XXVI, 1898, out of 100 grams of dried Bact. tuberculosis 

 secured the following substances: 



Nucleic acid (tuberculinic acid) 8.5 grams 



Nucleoprotamin 25.5 grams. 



Nucleoproteid 23.0 grams 



Albuminoids (keratin, etc.) 8.3 grams 



Fatty matter 26.5 grams 



Ash 9-2 grams 



!l Nishimura, Arch. f. Hyg. XVIII, 318, 1893, reports the finding of 0.17 per cent, xanthin, 

 0.08 per cent, adenin and 0.14 per cent, of guanin in his water bacillus. 



Vaughan, V. C. and associates, loc. cit., have noted the presence of certain diamino and 

 monamino acids. 



