284 MAURICE COLE TANQUARY. 



chjefly upon the final result which, after all, is the important 

 thing. 



APH/ENOGASTER TENNESSEENSIS. 



As this species does not occur in eastern Massachusetts I 

 collected part of a large colony from a wood near Urbana, 111., 

 June 8 and brought it with me a few days later to the Bussey Insti- 

 tution, where I transferred the ants to a plaster of Paris nest, 

 made on the Fielde pattern. The nest contained only workers 

 and a large number of larvae. By the middle of July winged 

 females began to appear and during the summer more than a 

 hundred were produced. These, and a few sent me from Illinois 

 later in the summer by Messrs. Hugh Glasgow and R. D. Glasgow, 

 were the ones used in the experiments. Altogether I tried thirty- 

 five queens of A. tennesseensis with eleven different colonies, two 

 of which were A .fulva subspecies aquia var. picea, one A. fulva, and 

 the others A. fulva aquia. The latter is probably more common 

 where A. tennesseensis occurs. Of these thirty-five queens I 

 got but one clear case of adoption and unfortunately this was with 

 one of the colonies for which I have but scanty notes. The notes 

 of this experiment are as follows: 



EXPERIMENT B. 240. 



Aug. 18. 3.00 P.M. I place an artificially dealated queen of Aphcenogaster 



tennesseensis in a Petri dish with five workers and about f f ty pupae of A . 



fulva aquia. 



Aug. 15 9.00 A.M. Queen standing by herself. 

 Aug. 20 9.30 A.M. The same. 

 Aug. 21 11.30 A.M. She is standing on the pile of pupae with the workers, none 



attacking her. 

 Aug. 22. The same. 

 Aug. 23 8.30 A.M. She stays in the midst of the workers and pupae and seems 



to have been adopted. 



Aug. 23 3.30. Still with the workers they never attack her. 

 Aug. 24 8.00 A.M. In the center of the bunch of workers; about three dozen of 



the pupae have become callows. 

 Aug. 28. Still being treated as their own queen. 

 Sept. 10. I have examined her every day up to the present time, and have never 



seen her treated by the workers other than as their own queen. There are 



now about sixty workers. 



In all the experiments with Aphcenogaster tennesseensis the 

 queen was attacked by the workers, usually at the very outset, 

 sometimes not until she had been in the nest for a few minutes. 



