384 OTTO GLASER. 



Asterias sperm, and Asterias secretion has the same effects on 

 Arbacia sperm. 



4. Paralyzed sperm may be reactivated but not reagglutinated. 



5. The egg secretions test negatively for reducing substances 

 and do not give the usual protein tests although they were found 

 to be faintly positive to the acid tests and the xanthoproteic. 

 This may be due to traces of the egg jelly. 



6. Agglutination may be gotten with dry egg powder. 



7. The agglutination reaction very possibly depends on a 

 surface effect. 



8. More soluble substances escape from the egg of Arbacia in 

 hypertonic sea-water and in sea-water infected with sperm, than 

 from unfertilized eggs in normal sterile sea-water in the same 

 length of time. 



9. The egg-secretion in certain concentrations retards develop- 

 ment measurably. 



10. 1.75 c.c. N f io NaOH added to 100 c.c. of sea water does 

 not accelerate the early cleavages. The retardation noted may 

 well be within the limits of error. 



1 1 . NaOH in the same concentration does markedly accelerate 

 the development of blastulse into plutei. 



12. Egg secretion in the concentrations employed + NaOH in 

 the concentration given above, results in a more marked re- 

 tardation of cleavage than the egg-secretion without the 

 NaOH. 



13. Egg-extract, as contrasted with egg-secretion, in addition 

 to retarding development, in a similar manner when employed 

 in a similar manner, results in cytolysis, arrests of development 

 in the early cleavages, and a general failure of the eggs to get 

 beyond the early non-motile blastula. 



14. The heightened rate of oxidation in the fertilized egg; the 

 increased rate of secretion in eggs undergoing fertilization; the 

 decreased volume after fertilization all point toward the pos- 

 sibility that initiation of development depends upon the removal 

 of substances, directly or indirectly antagonistic to oxidation. 

 Proof that the egg-secretion in certain concentrations measurably 

 retards development is however insufficient evidence either. for 

 the conclusion that it itself is the antagonist, or contains it. 



