180 COLLECTED STUDIES IN IMMUNITY. 



even in amounts up to 1 or 2 cc. was able to prevent solution, although 

 only the single solvent dose of phrynolysin was added to the mix- 

 ture of blood and serum. 



IMMUNIZATION WITH PHRYNOLYSIN. 



In order to furnish conclusive proof that phrynolysin is a true 

 toxin, a number of rabbits were immunized with the same. The 

 poison was injected subcutaneously, commencing with cc. and 

 increasing to 5 cc. in the course of eight days. The dose of 5 cc. 

 was then injected every 5 to 6 days for two or three times so that 

 in the course of three weeks about 30-35 cc. had been given. 



It is not advisable to give more than 5 cc. at once because other- 

 wise the animals die in one or two days. The anatomical findings 

 in an animal which has died of toad poison are negative, excepting 

 a marked hyperaemia of the abdominal viscera; no macroscopical 

 changes of the organs are demonstrable. 



As already mentioned, normal rabbit serum does not contain 

 a trace of anti-body against phrynolysin. The production of anti- 

 toxin commences about fourteen days after the injection of the toxin 

 and reaches its maximum in three weeks. Of the strongest serum 

 which I obtained, 0.025 cc. protected against double the solvent 

 dose of phrynolysin for 1 cc. 5% sheep blood. 



LITERATURE. 



VTTLPIAN, Comp. rend, de la soc. de biol., 1854, p. 133; 1856, p. 124. 



DOM. JORNARA, Sur les effets physiol. du venin de crapaud., Journ. de Therap., 



4, p. 833 et 929. 



G. CALMELS, Comp. rend., 98, 536 (1883), and Archiv. de physiol., 1883. 

 CAPPARELLI, Archiv. ital. de biol., 4, 72 (1883). 

 ROBERT, Sitzungs-Bericht der Dorpater Naturforschenden Gesellschaft, 9, 63 



(1891). 

 PHISALIX and G. BERTRAND, Toxische Wirkung von Blut und Gift der gemeinen 



Krote, Comp. rend., 116, 1080-1082, Arch, de physiol., 25, 511, and 517, 



Comp. rend. soc. biolog., 45, 477, 479. 

 D. JORXARA and CASALI, Das Gift der Krote und das Bufidin, Revista clinica 



Bologne, 1873. 

 A. PUGLIESE, Die Methsemoglobinbildende Wirkung des Krotengiftes, Archiv. 



di farmac. e terap., 1898. 

 EHRLICH and MORGENROTH, Uber Haemolysine, Berl. klin. Wochenschr. 1899, 



Nos. 1 and 22; 1900, Nos. 21 and 31; 1901, Nos. 10, 21, and 22. 



