THE H.EMOLYTIC PROPERTIES OF ORGAN EXTRACTS. 279 



decanted fluid mixed with 0.05 cc. of guinea-pig blood freed from 

 serum. (See Table VII.) 



TABLE VII. 

 GUINEA-PIG BLOOD (1 cc. 5%). 



We see, therefore, that at the single solvent dose has been com- 

 pletely anchored by the blood-cells and that after centrifuging this 

 leads to complete solution at higher temperatures; double the solvent 

 dose is still completely anchored by the blood-cells. This condition 

 of affairs does not at all correspond to the behavior of the complex 

 haemolysis of serum. 



It still remained to study another fundamental characteristic, 

 namely, the formation of antibodies. 



We made peritoneal injections into rabbits, using for this purpose 

 a, strongly active extract of ox pancreas that had been sterilized by 

 heating to 60 C. for one hour. The precipitate which developed being 

 regarded as the true active constituent, the mixtures were thoroughly 

 shaken and the whole injected. Two rabbits received 20 cc., 45 cc., 

 and 60 cc. of the extract at suitable intervals and were bled ten days 

 after the last injection. The antihaemolytic action of the serum against 

 the extract was found to be exactly the same as that of normal rab- 

 bits. (See Table VIII.) 



As can be seen from this experiment (the result of which is con- 

 firmed by a number of similar experiments with the serum of other 

 rabbits and of a goat treated in like manner) it has not been possible 

 to produce antibodies by injections of pancreas extract. 



The experiment, moreover, shows that normal rabbit serum already 

 possesses a marked inhibiting action on the haemolysis through organ 

 extracts. 1 W T e have been able to demonstrate this on all the species 



1 This action of the serum must always be taken into account in the ex- 

 periments, and the blood-cells first washed. 



