DISSOCIATION IN THE TOXIN-ANTITOXIN COMBINATION. 667 



subject, we have felt it advisable to publish the results of our experi- 

 ments, especially since they shed some light on the cause of the 

 paradoxical results. 



The botulism toxin and its antitoxin was kindly furnished us by 

 Professor Forssmann of Lund. We began by experimenting with 

 mice, and first determined the lethal dose by subcutaneous injections. 

 This is shown in the following table: 



TABLE I. 



* t2, etc., denote death on the second day, etc. 



We next determined the L* quantity of the antitoxin, using 

 1,000 times the fatal dose (0.1 cc.) for this purpose. The mixtures 

 of toxin-antitoxin were allowed to stand for three hours at room 

 temperature previous to injection. The result of this test is shown 

 in Table II. 



TABLE II. 



The experiments proper began with a mixture of toxin, 0.1 + 

 antitoxin, 0.0006. The mice were injected subcutaneously with 

 1/1, 1/2, 1/5, 1/10, etc., of this mixture. The dilutions were pre- 

 pared immediately before the injection, and the volume of the fluid 

 injected was always 1 cc. The result is shown in Table III. 



