STUDIES ON H.EMOLYSINS. 



Ill 



TABLE X. 



Activating Serum. 



Guinea-pig serum. 

 Rabbit serum. . . . 



Rat serum 



Goose serum 



Chicken serum. . . 

 Goat serum. ..... 



Pigeon serum. . . . 



Horse serum l . 



Amount of Immune 

 Body with which Com- 

 plete Solution Occurs. 

 cc. 



0.0025 

 0.005 

 0.005 

 0.015 

 0.015 

 0.05 

 no complement action 



1 This horse serum, which had been freshly obtained, failed also to reacti- 

 vate the immune bodies of a goat and a goose which had been immunized with 

 ox blood. Yet it was not at all free from complement, for even in amounts 

 of 0.15 cc. it dissolved guinea-pig blood completely. It did not act on rabbit 

 blood. 



This shows that when different sera are used as complements 

 there is a great variation in the amount of immune body necessary 

 for solution. Especially the extreme cases make it seem probable 

 that we are dealing with different types of partial-immune bodies, 

 to which different complements in the serum of the individual species 

 correspond. That the complements of different species are not iden- 

 tical is admitted even by Bordet, although he recognizes only a single 

 complement for each species. 



That these complements are anchored to the corresponding 

 immune body by means of a haptophore group may practically be 

 regarded as proven, (1) by our experiments with blood-cells which 

 had been laden with immune body, and (2) by the demonstration 

 of anti-complements which diverted the complements from the 

 immune body. 



According to our view the point at which the haptophore group 

 takes hold is situated in the complementophile portion of the immune 

 body. Hence we formerly designated the latter as " interbody " ; 

 recently we term it " amboceptor." A number of special investi- 

 gators have accepted this view, as can be seen from the designations 

 used by them ; thus P. Miiller, " copula " ; London, " desmon " ; 

 Metchnikoff: " cytase " = complement ; " philocytase " = immune body. 



Consequently we arrive at the view that in the mixture of immune 

 bodies in the case under discussion a number of different complemen- 



