204 



COLLECTED STUDIES IN IMMUNITY. 



therefore confirms the separation effected by means of the stromata, 

 whereby the complements of the normal haemolysins I and II are 

 separated from the rest. 



Bordet himself, by the way, has described such a case concerning 

 the combination rabbit blood guinea-pig serum. This experiment, 

 of course, was not to be reconciled with his Unitarian view, and he 

 therefore sought to explain this inconvenient result in accordance 

 with his view by assuming a special law of distribution for the 

 normal hsemolysins, together possibly with an inhibiting action 

 exerted by the products of the destruction of the red blood-cells 

 first used, on further solution of the same. 1 Against this we should 

 like to emphasize that in our case the result has been confirmed by 

 the experiment with blood stromata. By means of this, since the 

 stromata plus the anchored complement is removed by centrifuging, 

 Bordet's assumptions can be entirely excluded. 



Our absorption experiments therefore show that of the two possi- 

 bilities, namely, of a complement with several different zymotoxic groups, 

 or of a plurality of different complements, the latter assumption must 

 be accepted. 



Regarding the number of complements to be assumed for normal 

 goat serum, as based on our experiments, this can best be seen from 

 the following table: 



TABLE VI. 



1 This objection, moreover, is entirely incomprehensible to us. According 

 to our view, normal and artificially produced hapmolysins manifest their action 

 by means of the same mechanism; for when the normal amboceptors are re- 

 placed by the host of amboceptors present in an immune serum, new comple- 

 mentophile groups come into action, and with these, of course, new partial 

 complements. 



