PRODUCTION OF H.EMOLYTIC AMBOCEPTORS. 



247 



TABLE II. 

 1.0 cc. 5% Ox BLOOD. 



A. Blood + amboceptor are kept at 37 C. for one hour. After centrifuging 

 the fluid is decanted and the sediment mixed with 2 cc. physiological salt 

 solution and 0.2 cc. rabbit serum as complement. 



Complete Haemolysis. 



Serum rabbit I .05 cc. 



II 0.05 " 



III 0.25 " 



B. BLOOD+ AMBOCEPTOR +0.1- 0.2 GUINEA-PIG SERUM AS COMPLEMENT. 



Serum rabbit IV 0.1 cc. 



" " V 0.05 " 



" " VI 0.05 " 



" " VII 0.028 " 



" " VIII 0.013 " 



" " IX more than 0.25 " 



" " X 0.05 " 



" " XI lessthanO.05 " 



serum does not suffice to overcompensate the auto-anticomplement 

 present. For example, the serum of rabbit III shows the following 

 solvent action after the addition of 0.6 cc. rabbit serum: 



0.5 cc 0.075cc very little 



0.25" trace 0.05 " " " 



0.15" " 0.025" trace 



0.1 " very little 



The abnormal course of this slight haemolysis shows very well 

 the interference of anticomplement on the one hand and of the 

 amboceptor on the other. 



The similarity of the amboceptor produced by injections of goat 

 serum to that produced by injections of blood is more plainly seen 

 by the fact that the anti-immune body obtained by immunization 

 acts against the former amboceptor just as well as against the latter. 

 Table III shows this behavior very well. 



The anti-immune body used was contained in the inactivated 

 serum of a goat which had been injected several times with the serum 

 of rabbits immunized with ox blood. 0.3 cc. of this anti-immune 

 body serum were mixed with varying amounts of the amboceptor sera 

 to be tested and the mixtures kept at room temperature for one hour. 

 Thereupon 1 cc. of a 5% suspension of ox blood-cells was added to 



