EARLY DEVELOPMENT OF GRAFFILLA. 1 75 



leased out and the specimens again washed in water, with 

 the rr-nlt that thirty-eight Graffillcc were -ecuivd. I'ndoubtcdlv 

 many individual parasites escape from tin- kidiu-y of the ho-t and 

 latrr found in the mantle cavity and on the pill-, and this 

 would .Kiount for their discovery there by Linton. Further- 

 nion- tin- method ordinarily employed in opening the niolli. 

 \\oiild in e--arily result in injuring the kidney, and thu- |u rinit 

 the < -( ape of the parasite from that organ. Th< iinnit 



mentioned above would seem to indicate clearly that G. ^fnn-I- 

 li()nr,i i- a true endoparasite, but the experiment \\a- performed 

 .it tin- c|o-i- of the season and the opportunity \\a- IK>I ofieivd 

 to -i -i ilt- the <|iiestion conclusively, as that could onl\ In done by 

 in. tkin. I'ul dissections of the individual mollu-i -. \\ V 



-hould expect to find this species of parasite in the kidney or li\ er 

 oi i IK ho-t -ince all of the other species of the genii ''ilia 



are found in the same organs of the various mollu- 



The In -i M ,i>on of the year in which to secure ( llifmni 



.n \\<H,d- ||..|r is during August, from the loth to ihe joih of 

 ilie nioiiili. ^I'ecimens may be obtained prior to \\i\--, but iliev 

 .in u^u.ilK iininobile individuals which contain nuinerou-, \oiin- 

 thai lia\ e libel. iti-d themselves from their capsule- and . m- -\\ini- 

 iniii'c about in the mescnehyme. Such material i- valuable lor 

 obtaining \ei\ \ounganimals. On July 5. 1911, several of these 

 e\h. ui-ied niothcrs wiTi' placed separatel\- in lian-iii- drop- of 

 the iluid taken from the mantle cavity of Moiliolns. The cover 

 sli|> tioin \\hich the drop was suspended wa- placed abo\e the 

 cavity "t .1 hollow ground slide and sealed with \a-eliin-. In 

 ihi- \\a\- the -[leciniens could easily be studied under the ini- 

 croscope, < >n the following day it was noted that mo-i of the 



\-oiing ha<l ruptured the wall of the mother and were -\\inuniiiii 

 about in the drop. In one case the escape of tin- youn^ \\a^ 

 actualk ol,M-r\ed. \'oung animals secured by this method can 

 be kepi ali\e \\iihoiit much trouble for about two days, and un- 

 doubted lv \\ould li\'e longer if proper care \\ere taken. I lo\vc\ er, 

 it \\a- found unnecessary to obtain material for -tudy in this 

 \\.iv atier toii\-ci-ht hours, for the washings of Modinlns yield 

 manv young -pecimens that correspond in -i/e to ihe-e two-day 

 old \\ orm-. 



