^44 



KRXEST E. JUST. 



Watch Glass Series. 



A female was opened at 9:58, a male at 10:00. In five watch 

 glasses of india ink ground up in sea-water eggs and sperm were 

 mixed at intervals of two minutes. At 10:10 a few eggs were 



FIG. 7. Just after disappearance of sperm within the egg. 



inseminated in the ink solution on an uncovered slide (no. 6). 

 About two minutes after an insemination the jelly began to form; 

 in fifteen minutes the sperm indicator was well developed. Eggs 



FIG. 8. First polar body forming. 



inseminated at 10:15 in a watch glass (no. 7) were washed at 

 10:30: that is, when the indicator had reached its maximum of 

 development. 



