112 



SAKYO KANDA. 



The microscopic examination of the preparations showed that 

 the darker and heavier substances and crystal-like materials lay 

 in the extreme anterior end of the animal which was thrown away 

 from the axis of the centrifuge. Next to these came the micro- 





FIGS, i and 2. Camera drawings of Paramecium caudatum. A, anterior end; 

 P, posterior end. 



nucleus and then the macro-nucleus. The chromatin w r hich was 

 stained green, seemed to have been precipitating to the outer 

 nuclear wall, since this part of the nucleus was stained much 

 darker than the inner, as Figs, i and 2 have shown. The "plas- 

 mosomes" and cell granules were stained orange, but the cilia did 

 not show very distinctly. The writer thus found in confirmation 

 of Lyon's and McClendon's results that Paramecium contains 

 protoplasmic materials of differing specific gravities. Figs. I 

 and 2 show specimens drawn with the aid of a camera attach- 

 ment. 



That the anterior end of the animal, when centrifuged, was 

 thrown away from the axis of the centrifuge presumably on ac- 



