THE MICROVIVISECTION METHOD. 



123 



tion of his technique and is of the utmost value for the prospective 

 worker in micro-dissection. As he developed his method prin- 

 cipally for the use of pipettes and also because his latest paper 

 appeared in a publication not readily obtainable, I have en- 

 deavored to give here a description of the method with modifica- 

 tions and new developments in the application of the method to 

 micro vivisection. 1 



A simple form of Barber's apparatus is shown in Fig. I. The 

 moist chamber, which is open at one end and with sides from 8 to 



Clompfor 

 Needle 



Front and baoK 

 move men 



Side to side 

 movement" 



n.c 



FIG. i. Barber's single three-movement pipette holder, glass needle and moist 

 chamber arranged to illustrate method of dissecting cells in a hanging drop under 

 the highest magnification of the microscope. (Substage of miscroscope omitted 

 in drawing.) 



1 In 1912, G. L. Kite first applied Barber's apparatus to microdissection, which 

 quickly resulted in the publication of several papers. Kite was to have prepared 

 an article on the method when his health unfortunately broke down. Publications 

 on microdissection have appeared as follows: 1912, Kite (on the fertilization 

 membrane) in Science, N. S., XXXVI.; Kite and Chambers (on chromosomes and 

 nucleus) in Science, N. S., XXXVI. 1913, Kite (on permeability of cells) in BIOL. 



