THE MICROVIVISECTION METHOD. 



129 



Tissue cultures are grown on. square coverslips. The slips 

 are then placed on the moist chamber for microdissection, the 

 parts of the chamber not roofed over being previously covered 



with slips of glass. 



THE NEEDLES. 



These are made from glass tubing with an outside diameter of 

 about 4-6 mm. and a lumen of 3-5 mm. The technic is readily 

 mastered with soft glass tubing but needles made of hard glass 

 are more durable. 



FIG. 5. Method of making the capillary pipettes and microdissection needles. 



The needle points are made over a minute flame. A service- 

 able micro-burner with an acetylene generator is shown in Fig. 5. 

 The microburner is made out of a piece of hard glass tubing bent 

 almost at right angles and with one end closed except for the 

 smallest possible aperture that will retain a flame. This is done 

 by heating the end and pinching it with a pair of forceps. 

 When set up the tip of the burner should be at a height of about 

 5-6 cm. above the surface of the table. The size of the flame 

 is regulated by the screw pinch-cock set on the rubber tube con- 

 necting the burner with the gas generator. 



