CYTOPLASM OF ANIMAL AND PLANT CELLS. 3 



Acid violet: Windsor bean root-hairs, Saprolegnia, and Mucor. 



Beibricher scarlet: Barley root-hairs, the immature eggs of 

 Necturus, the peritoneal epithelium of Necturus, Mucor, Sapro- 

 legnia, and Paramecium. 



Indigo-carmin: Saprolegnia and Mucor. 



Ponceau, P. R.: the root-hairs of the Windsor bean, Ameba 

 proteus, Paramecium, Saprolegnia, and Mucor. 



Indulin: root-hairs of Windsor bean, Ameba proteus, Sapro- 

 legnia, and Mucor. 



Nigrosin: the peritoneal epithelium and the very immature 

 eggs of Necturus, and Mucor. 



Eosin: Windsor bean root-hairs, Ameba proteus, Paramecium 

 Mucor, and Saprolegnia. 



In recent papers Loewe 1 states that even basic dyes are ad- 

 sorbed by lipoids, when added to solutions of lipoids in organic 

 solvents. 



THE INTRACELLULAR INJECTION OF DYES AND CRYSTALLOIDS. 

 If indigo-carmin, methyl red, trypan blue, thiocarmine R., or 

 azolitmin dissolved in sea-water be injected into any portion of 

 the cytoplasm of the starfish egg, the sea water slowly diffuses 

 into the surrounding protoplasmic gel, and finally the granular 

 precipitated dye alone remains. An injection of indigo-carmin 

 into the cytoplasm of an immature egg of Necturus results in a 

 slight staining of the wall of the vacuole, while an injection of such 

 widely different dyes, as indigo-carmin, trypan blue, and janus 

 green (diethyl-safranin-azo-dimethyl-anilin), into the cytoplasm 

 of the striped muscle cell of this animal results at most in a 

 localized staining of the cytoplasmic gel immediately surrounding 

 the mass of injected dye. The injection of indigo-carmin or 

 nigrosin into the vacuole of Spirogyra results in a blue-green or 

 light violet staining, respectively, of the entire cytoplasm. A 

 blue-green staining of the cytoplasm of Hydrodictyon is affected 

 by an intravacuolar injection of indigo-carmin. In fact, every 

 acid dye that was injected into the vacuole of Spirogyra, Hydro- 

 dictyon, the leaf cells of Elodea, and the parenchyma cells of 

 Tradescantia penetrated, and stained the cytoplasm and usually 

 the nucleus. 



1 Loewe, Biochemische Zeitschrift, 1912, X'LIL, p. 150. 



