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NEIL S. DUNGAY. 



possible trace of alcohol carried over with the sperm in the process 

 of insemination, was made by adding alcohol to the control 

 cultures at the time of insemination. The addition in quantities 

 comparable to the amount present in the experimental cultures 

 was without effect. Table II. gives the principal features of a 

 representative experiment, the minor features being omitted. 



TABLE II. 



EXPERIMENT A 2. July 4, 1911. Sperm removed at 9:00 A.M. from a male 

 with testes about half soft. Two drops of thick sperm placed in 10 c.c. of each 

 grade of alcohol at 9:15 A.M. and left there 5 hours and 20 minutes. Eggs about 

 2 per cent, immature. Used immediately after removal. All except A 2.0 (the 

 uninseminated control) inseminated at 2:35 P.M. Discontinued July 7, 1911. 



V. CYTOLOGICAL STUDY. 



A. Normal. 



I have found it necessary to go into some of the cytological 

 details which are to be found in the egg of Nereis after insemina- 



