590 Annals of the South African Museum. 



is occupied by the hollow centre ; hence, the enlargement is far less 

 than in the case of the asexual daughter colony and the resultant 

 cells at any stage consequently far smaller. 



The total number of divisions is nearly always nine, resulting in a 

 hollow oblate spheroid, more or less kidney-shaped, as seen in side 

 view, nearly circular in polar view, composed of some 512 cells. 

 Very occasionally only eight divisions take place, giving half-size male 

 bundles. 



From the 16- to the 64-celled stage the structure appears as a wide- 

 mouthed bowl (fig. 5, J, K ; Plate XLII, L, M), the mouth of which 

 gradually grows smaller in succeeding divisions. As a result of the 

 shifting of the polar axis the protoplasmic strands connecting the 

 developing male bundle to the parent are not attached round the 

 phialopore, as in the case of the asexual daughter, but to one side of 

 the colony, round the outer end, thus leaving the lip of the phialopore 

 entirely free. The connecting strands show well in figs. H, K, L on 

 Plate XLI. 



At completion of division the globoid lies inside its vesicle within 

 the hollow of the parent, its long axis more or less radial to the 

 parent (Plate XLII, N) ; the cells are similar in form to those of the 

 daughter colony, but smaller. Each contains a single pyrenoid in 

 a deep green chloroplast lying adpressed to the outer surface of the 

 cell, the inner clear end containing the nucleus. The phialopore is 

 small and rounded in outline (fig. 5, L). Mucilage strands extending 

 across the central hollow between the cells can often be seen even in 

 living material, and more clearly in stained microtomed sections. 



3. Period of Re-orientation of the Sperm Bundle. 



(a) Preparation. Owing to the small size it is more difficult to 

 watch the changes in cell form than in the case of the daughter colony. 

 Here, as there, the first signs of preparation for inversion are most 

 clearly seen at the phialopore. The lip becomes depressed, curling 

 inwards into the hollow of the spheroid, until it may almost reach the 

 centre (fig. 6, A ; Plate XLIII, A, B ; Plate XLIV, D), hence it is 

 accompanied by a widening of the pore, as seen in surface view. The 

 denting so characteristic of this stage in the daughter colony is not 

 seen ; evidently " denting " is correlated with the much greater size 

 of the daughter. But the change in the shape of the cells soon 

 becomes very apparent the outer wall is rounded, the long axis is 

 now radial, and the clear inner apex more pointed and well defined. 



