CHARTS AND THEIR EXPLANATIONS. 



95 



Chart XXX (rat 71, male) shows long-continued feeding of isolated 

 foodstuffs and also long-continued maintenance on glutenin from wheat as the 

 onlyprotein. The history of the animal is on p. 59. The diets were as follows: 



Constituents. 



Glutenin 



Casein 



Starch 



Sugar 



Agar 



Salt mixture I 

 Lard 



Per. 1. 



Per. 2. 



Pei . 3. 



p. ct. 



16.36 

 0.0 



22.27 



13- 63 

 4-54 

 2.27 



40.9-3 



Per. 4. 



p. ct. 



18.0 

 0.0 



14-5 



ISO 

 SO 

 2.5 



450 



Periods 

 5 and 8. 



p. ct. 

 18.0 



0.0 

 34-5 

 20.0 



5.0 



2.S 

 20.0 



Constituents. 



Glutenin 



Edestin 



Starch 



Sugar 



Agar 



Salt mixture I. 

 Lard 



Constituents. 



Glutenin 



Protein-free milk . 



Starch 



Agar 



Lard 



Chart XXX further shows the possibility of maintaining an animal 

 satisfactorily under our cage conditions for 458 days. Attention is particu- 

 larly directed to period 9, during which the only change in the diet consisted 

 in substituting protein-free milk for some of the non-protein components 

 of the dietary. The lowest line represents the nitrogen balance of the rat. 



Chart XXXI. 



Chart XXXII. 



terminated 



220 



200 



Chart XXXI (rat 222, male) shows early growth curve of male on milk 

 diet, having the following composition: Trumilk, 60.0 p. ct.; starch, 15.7 p. 

 ct. ; salt mixture I, 1.0 p. ct. ; lard, 23.3 p. ct. 



Chart XXXII (rat 195, male) shows normal growth curve of male on 

 milk diet, having the following composition : Trumilk, 60 p. ct. ; starch, 16.7 

 p. ct.; lard, 23.3 p. ct. 



