IOO 



FEEDING EXPERIMENTS WITH ISOLATED FOOD-SUBSTANCES. 



Chart XL. 



20 



A0 



60 



80 



100 



120 140 



Days 



160 



180 



200 



220 



240 



260 



Periods 2 and 3. 



Chart XL (rat 150, female) shows the influence 

 of a diet containing a mixture of isolated foodstuffs 

 in which casein was the sole protein. Period 1 repre- 

 sents the normal growth of the animal on a mixed 

 food. The casein feeding began with period 2. The 

 influence of faeces of normally fed animals in prevent- 

 ing decline in body- weight is shown during period 3. As shown by the food 

 intake the favorable effect is not due to an increased consumption of food. 

 The diet during period 1 consisted of mixed food; during periods 2 and 3 as 

 shown herewith. 



p. ct. 



Casein 18.0 



Starch 32. 5 



Sugar 21. 9 to 26. 9 



Agar 0.0 5.0 



Salt mixture I 2.5 2.6 



Lard 20.0 25.0 



Chart XLI (rat 127, male) shows the influence of a diet containing a 

 mixture of isolated foodstuffs in which casein was the sole protein. Period 1 

 represents the normal growth of the animal on a mixed food. The casein 

 feeding began with period 2. The influence of fasces of normally fed animals 

 in preventing for a time the decline in body-weight is showm during period 3. 

 Period 4 shows the favorable nutritive influence of the substitution of 

 protein-free milk for a part of the non-protein constituents of the diet. The 

 diet during period 1 consisted of mixed food. During periods 2, 3, and 4 

 the composition of the food was as shown herewith. 



