ii4 



FEEDING EXPERIMENTS WITH ISOLATED FOOD-SUBSTANCES. 



Chart LXXV. 



Chart LXXVI. 



p. ct. 



Edestin 18.0 



Protein-free milk . 28 . 2 



Starch 23.8 



Agar 50 



Lard 25 . 



Charts LXXV (rat 248, female) and LXXVI (rat 

 253, female) show growth from an early age on a diet 

 containing protein-free milk in which edestin formed the 

 sole protein. It should be noted that the animals in 

 growing to several times their original weight must have 

 synthesized their purine- and phosphorus-containing complexes from purine- 

 free food. The influence of size on the food requirement is shown by the 

 food-intake curve. The diet was as shown herewith. 



Chart LXXVII (rat 114, male) shows the failure of edestin (period 2) 

 to maintain previous satisfactory nutritive condition of the animal during 

 period 1, on mixed food, even after adding faeces to the diet (period 3). 

 Immediate improvement and satisfactory nutritive condition followed addi- 

 tion of protein-free milk to edestin food (period 4) . The diet consisted of 

 mixed food for period 1, and for periods 2,3, and 4 was as shown in table. 



Constituents. 



Per. 2. 



Per. 3. 



Chart LXXVIII (rat 140, female) 

 shows the failure of maintenance on a diet 

 in which edestin formed the sole protein 

 (period 2), until protein-free milk was 

 added to the diet (period 3). Period i, 

 on mixed food, is introduced to show the 

 previous satisfactory nutritive condition 

 of the animal. The diet consisted of 

 mixed food for period 1, and for periods 2 and 3 it was as shown in table. 



p. ct. 



Edestin 18.0 



Protein-free milk ... . 0.0 



Starch 29 . 5 to 32 . 5 



Sugar 15.0 17.0 



Agar . 



Salt mixture I . 



Lard 



5.0 



2.5 

 25. 



30.0 



p. ct. 



18.0 

 28.2 

 23.8 



0.0 



5-0 



0.0 

 25.0 



