CHARTS AND THEIR EXPLANATIONS. 



117 

 Chart LXXX. 



120 

 300 

 280 

 260 

 240 

 220 

 200 

 180 

 160 

 140 

 120 

 100 

 80 



60 



E 

 a 



u 

 O 



20 



40 



60 



80 



100 



120 140 



Days 



160 



180 



200 220 240 26C 



Chart LXXX (rat 148, male) shows the failure of maintenance on a 

 diet in which edestin formed the sole protein (period 2), until protein-free 

 milk was added to the diet (period 3). Period 1, on mixed food, is intro- 

 duced to show the previous satisfactory nutritive condition of the animal. 

 Note the influence of changes in diet on the food consumption. The diet 

 consisted of mixed food for period 1 , and for periods 2 and 3 was as follows : 



Constituents. 



Per. 2. 



p. ct. 



Edestin 18.0 



Protein-free milk .... 0.0 



Starch ' 20 . 5 to 32 . 5 



Sugar I 15.0 17.0 



Agar J 5-0 



Salt mixture 1 2.5 



Lard 25.0 30.0 



Per. 3. 



p. ct. 



18.0 



28.2 



23.8 



0.0 



5-0 



0.0 



25.0 



