130 FEEDING EXPERIMENTS WITH ISOLATED FOOD-SUBSTANCES. 



Chart CXV. 



250 



240 

 220 

 200 

 180 



160 



140 



120 



100 



BO 



60 



40 



20 



40 



60 



80 



100 



120 140 



Days 



160 



180 



200 



220 



240 



26! 



Charts CXV (rat 144, male) and 

 CXVI (rat 134, female) show the failure 

 of animals previously well nourished on 

 a mixed diet (period 1), to be main- 

 tained on a diet in which gliadin formed 

 the sole protein (period 2), until pro- 

 tein-free milk was added to the food 

 (period 3). The food during period 1 

 was mixed. During periods 2 and 3 it 

 was as shown herewith. 



Constituents. 



Per. 2. 



p. cl. 



Gliadin (from wheat). .. 18.0 



Protein-free milk 0.0 



Starch 29 . 5 to 34 . 5 



Sugar 15.0 17-0 



Agar S 



Salt mixture I 2.5 



Lard 25.0 30.0 



Per. 3. 



p. ct. 

 18.0 

 28.2 

 20.8 



CO 



5.0 



28.0 



Chart CXVII (rat 129, female) shows the main- #.*. 



, v , . , . . l r Gliadin (from wheat)... 18. 



tenanee in period 2 on a diet containing protein-lree protein-free milk 28.2 



milk and gliadin as the sole protein. Note that the f^* 1 / ; ;;:;;;;;;:;::;; "; J 

 animal did not decline like those fed on gliadin with- Lard. . . ... 28.0 



out protein-free milk. The preliminary period 1 on 



a mixed diet, during which the animal was twice pregnant, is introduced to 

 show the excellent previous nutritive condition of the rat. The composition 

 of the food for period 1 was mixed; for period 2 it was as shown herewith. 



