294 



CRYSTALLOGRAPHY OF THE HEMOGLOBINS 



WILD CAT OK BAY LYNX, Lynx rufus. Plates 90, 91, and 92. 



The specimen of blood was received from the National Zoological 

 Park at Washington, District of Columbia. The blood, which had been 

 collected in oxalate, was dark and thick. It was laked with ether and 

 centrifugalized for several hours; and from the clear solution the slide 

 preparations were made as usual. The crystals of reduced hemoglobin 

 formed very readily, but negatives were not made until the following day, 

 by which time some crystals of oxyhemoglobin had appeared in the form 

 of very thin plates and also in the form of prismatic crystals. The crystals 

 kept well, the reduced-hemoglobin crystals especially being quite insoluble; 

 and after several days they were rather sharper and more perfect than at 

 the end of the first 24 hours. The crystals of oxyhemoglobin were found 

 rather sparingly in the slides, but developed in nearly all cases. They 

 showed three habits, of which the tabular type was perhaps the most 

 common. 



Oxyhemoglobin of Lynx rufus. 



Orthorhombic: Axial ratio 0.9866 : 1 : 0.3849. 



Forms observed: Unit prism (110), macroprism (430), brachydome (041), pyramid 

 (991), macropinacoid (100), brachypinacoid (010), basal pinacoid (001). 



Angles: Unit prism angle, 110 A 110=89 15'; macroprism angle, 430 A 430 = 

 73; brachydome angle, 041 A 031 = 114; pinacoids 100 A 010 = 90; pyramid edges 

 of (991), actual angle over pole =32 15'. 



343 



344 



346 



347 



c 



Fioa. 344, 345, 346, 347. Lynx rufia Oxyhemoglobin. 



Habit of the prismatic crystals a rather short prism (430) with the brachydome 

 (041) (text figure 344), the ratio of length to thickness (on b) about 3 : 1 or less. These 

 prismatic crystals are generally rather small, but are very numerous in the slides in which 

 they develop. The tabular crystals are flattened on the base and very thin; their 

 bounding planes are the unit prism (110) and the two vertical pinacoids (100) and (010), 

 with the pinacoids usually predominating (text figures 345 and 346) ; but sometimes 

 only the two pinacoids or the unit prism form the bounding planes, which makes a square 

 tabular crystal. The third type of crystal (text figure 347), which was observed in a 

 few slides only, resembled an isometric octahedron and appeared to be a mimetic twin 

 showing the prism faces only, the two interpenetrant prisms (430) being twinned on a 

 macrodome, but possibly it may be only the prism (430) and the dome (041) in equilib- 

 rium. The only angles that could be measured corresponded to the angle of the prism 

 of the ratio (430). Aside from the possible twin in these isometric-looking crystals, no 

 definite twins were observed in the oxyhemoglobin crystals. 



The color of these crystals was a bright scarlet, the usual color of oxyhemoglobin 

 when the crystals are not strongly pleochroic. Pleochroism was weak and hardly notice- 



