118 PREPARATION AND CRYSTALLOGRAPHY OF HEMOGLOBINS 



These figures do not agree with those of Zinoffsky for the hemoglobin of 

 the horse, or with those of Hiifner for the hemoglobins of the pig and ox. 

 The relation of Fe to S in clog's hemoglobin, Jacquet found to be 1 : 2.85; 

 Zinoffsky found 1:2 for the horse ; and Hiifner found 1:2 for the pig 

 and ox. Jacquet believes his sulphur value to be too small, and that 

 there is 1 atom of Fe to 3 of S. Later (Zeit. f. physiolog. Chemie, 1889, 

 xiv, 289) he analyzed crystals of the hemoglobin of the dog, which he 

 prepared by a modification of his previous process. The earlier method was 

 used, except that the solution of the corpuscles after the addition of the 

 ether was centrifugalized in a machine running at from 1,600 to 2,000 

 revolutions, whereby the stromata could be partly done away with. The 

 hemoglobin 3 times crj^stallized contained only a trace of phosphoric acid, 

 the quantity not being estimated. The mean of his analyses was 



54-57-^7. 22Ni6-38So-56sFe -336020-93 



The amount of water of crystallization was 11.39 per cent. The ratio of 

 Fe to S was 1 : 2.96, which was higher than in his preceding investigation. 

 From the values obtained he gives the formula C 7 5 8 H 1 203N 1 9 5 Fe0 21s , and the 

 molecular weight as 16669. 



He also analyzed the oxyhemoglobin of the chicken. In the prepara- 

 tion of the crystals he made a special effort to get rid of the large amount of 

 phosphoric acid (0.77 per cent) shown in the preparations of goose crystals 

 by Hoppe-Seyler. He found that he could not treat the blood in precisely 

 the same way as dog's blood, because when the corpuscles are agitated with 

 ether a gelatinous mass is formed which could not be filtered. The corpuscles 

 were therefore treated with an equal volume of water and one-third volume 

 of ether. The mixture when heated to 35 formed into dark-red, gelatinous 

 lumps which were separated from the fluid by centrifugalization. The clear 

 fluid thus obtained was readily filtered, and by the customary treatment 

 very soluble needles of hemoglobin were formed. By reciystallization both 

 rhombic plates and prisms were obtained. The three times crystallized 

 hemoglobin was found upon analysis to have the following composition : 



The water of crystallization was 9.333 per cent, and the ratio of Fe to 

 S 1 : 4.485. If the molecule be doubled the ratio is 2 : 9. In comparing 

 the analyses of the oxyhemoglobin of the dog, chicken, and horse, he states 

 that although these hemoglobins are different they have a similar iron 

 capacity, which warrants the conclusion that the iron-containing group in 

 the various hemoglobins is the same. Jacquet made ineffectual attempts 

 to prepare crystals from fresh salmon blood, but succeeded when the blood 

 was left to rot, there appearing clusters of crystals and beautiful single 

 rhombic prisms. 



Jolin (Archiv f. Anat. u. Physiologic, 1889, 265) records that the hemo- 

 globins of the dog and guinea-pig differ from that of the goose in their 

 absorptive rapidity in relation to O, as well as in the volume of O absorbed. 



The increased crystallizability of putrid blood has been noted by a 

 number of observers and referred to in previous pages, and Bond (London 



