206 CRYSTALLOGRAPHY OF HEMOGLOBINS OF THE UNGULATES. 



direction of less elasticity. Pleochroism is strong, parallel to the length of the fibers; 

 a is colorless, normal to the length, b and c are purple. In the spherulitic masses the 

 pleochroism shows strongly, dividing the spherulite into sectors, the opposite sectors 

 being of the same color; a colorless, c and & purple, as in the prisms. 



MDNTJAK, Cervulus munljak. Plates 36 and 37. 



The specimen of blood was received from the New York Zoological 

 Gardens and was in a somewhat putrid condition. The blood, containing 

 oxalate, was laked with ether and centrifugalized and from the clear solu- 

 tion slides were prepared in the usual manner. The blood crystallized 

 slowly and the crystallization was therefore carried on at temperatures 

 near C. The first crystals to form were oxyhemoglobin, short prisms 

 with very oblique terminations; later, crystals of reduced hemoglobin 

 formed in the shape of long square-ended rods or lath-shaped crystals, and 

 in curving arborescent forms. The crystals of the oxyhemoglobin with- 

 stood changes of temperature fairly well, but the crystals of reduced hemo- 

 globin were rapidly dissolved when brought into the warm room. 



Oxyhemoglobin of Cervulus muntjak. 



Monoclinic: Axial ratio a : b : 6 =1.303 :!:<!; /?=52. 



Forms observed: Unit prism (110), base (001). 



Angles: Traces of the prism angle on the base, or plane angle of 

 the basal section, edges 110-001 A 110-001=75 (105 normals), base 

 to prism edge=/? = 52, actual prism angle 110 A 110=91 30' (calcu- 

 lated 91 45'). 



Habit short prismatic, the unit prism terminated by the oblique 

 basal pinacoid; sometimes in equilibrium (text figure 153), and then 

 looking rather like a rhombohedron. The crystals grow isolated or 

 FIG. 153. Cervuiut munt- crowded together in great numbers, and sometimes appear to twin on 



jak Oxyhemoglobin. -j r J.T. ? v I. iifj 



a pyramid of the unit series or perhaps on a hemiorthodome. 



Pleochroism is rather marked; a nearly colorless, somewhat yellowish; & strong 

 red, c deep red. On all sections in the zone of (OOl)-(lOO) the extinction is symmetrical; 

 looking along the symmetry axis 6 the extinction angle is 30 with the prism edge. The 

 plane of the optic axes is the plane of symmetry, and the orientation of the elasticity 

 axes is as follows: a A a = 8, in the obtuse angle; b=&; c A (5=30, in the obtuse 

 angle. In convergent light, looking nearly along c, the biaxial interference figure was 

 seen with the brushes rather widely separated. The optical character is hence positive, 

 as the acute bisectrix is c. 



Reduced Hemoglobin of Cervulus munljak. 



Monoclinic: Axial ratio can not be determined, as only 

 the pinacoids arc developed. The angle /? seems to be 90. 



Forms observed; Base (001), orthopinacoid (100), clino- 

 pinacoid (010) 



Angles of orthopmacoids and clmopmacoids, 90; base Hemoglobin, 



to orthopinacoid about 90. 



Habit lath-shaped, flattened on (001) and elongated along a (text figure 154), 

 also hair-like, in feathery and arborescent groups and tufts; when broad lath-shaped, 

 the crystals compound on the base. The crystals melt or dissolve very rapidly on being 

 brought into a warm room. They are very much larger than the oxyhemoglobin crystals. 



Plcochroism is rather marked; a very pale bluish-lilac; b purplish, pale but stronger 

 than a; c deep reddish-purple. Extinction is straight on the flat and about 18 with the 



