STEWART'S DISEASE OF SWEET-CORN (MAIZE). 

 Table 19. Growth of Bacterium stewarti in Various Media. 



141 



Each tube was inoculated with 2 big 3 mm. loops of the cloudy fluid from a 6-day-old peptonized beef-broth culture. (From notes of 



August 17, 1908.) 



Medium. 



Stock 3410: cane-sugar, ammonium Galloway (2 tubes) 

 tartrate, water. 



Stock 3410 1 McCulloch 



Do. * ! Hedges (2 tubes) . . . 



Stock 341 1 : cane-sugar, ammonium Galloway (2 tubes) . 



lactate, water. 

 Stock 341 1 McCulloch (2 tubes) 



Do. 



Hedges (2 tubes) . 



Stock 3408: cane-sugar, asparagin, Galloway (2 tubes) . 



water. 

 Stock 3408 McCulloch (2 tubes) 



Do.. . Hedges (2 tubes). . . No. 1, Aug. 3. 



No. 2, Aug. 14. 

 Stock 3413: tomato juice, titrating Galloway (3 tubes) . No. i.Aug. 14. 



Date inoculated. Date reinoculated. 



No. I, Aug. 3. . Aug. 

 No. 2, Aug. 14. 



Same as above. 

 No. 1, Aug. 5. 

 No. 2, Aug. 14. 

 No. i, Aug. 3. 

 No. 2, Aug. 14. 

 No. 1, Aug. 3 . 

 No. 2, Aug. 14. 

 No. 1, Aug. 5. 

 No. 2, Aug. 14. 



No. 1, Aug. 3. 

 No. 2, Aug. 14. 

 No. 1, Aug. 3. 

 No. 2, Aug. 14. 



+60. 



Stock 3413. 

 Do.... 



Stock 3407: cane-sugar in water. 



No. 2, Aug. 3. . 

 No. 3, Aug. 3.. 

 McCulloch (3 tubes) Same as above. 

 No. 1, Aug. 5. . 

 No. 2, Aug. 14. . 



Hedges (2 tubes). 

 Galloway (2 tubes) 



Stock 3407. 

 Dot. 



Stock 3409: cane-sugar, ammonium 



citrate and water. 

 Stock 3409 



Do 



Stock 3406: sy-ntheticj 



Stock 3406 



Do 



Stock 3415: beef-bouillon +73 



Stock 3415 



Do 



Stock 3424 : beef-bouillon 3 5 



Stock 3424 



Do 



Stock 3427: Dunham's solution 

 with methylene blue. 



McCulloch (2 tubes) 

 Hedges (2 tubes) . . . 



Galloway (2 tubes) . 



McCulloch (2 tubes) 

 Hedges (2 tubes). . . 

 Galloway (2 tubes) . 

 McCulloch (2 tubes) 

 Hedges (2 tubes)... 

 McCulloch (3 tubes) 

 Hedges (3 tubes). . . 

 Galloway (3 tubes) 

 McCulloch (3 tubes) 

 Hedges (3 tubes).. . 

 Galloway (3 tubes) . 

 3 tubes, one of each 

 strain. 



No. 1, Aug. 3. 

 No. 2, Aug. 14. 



No. 1, Aug. 3. 

 No. 2, Aug. 14. 

 Same as above. 



No. 1, Aug. 3. 

 No. 2, Aug. 14. 

 Same as above. 



.... Do 



....Do 



....Do 



...Do 



Aug. 15 (a. m.). 



.... Do 



....Do 



... .Do 



....Do 



.... Do 



....Do 



M 



Aug. 14. 



Aug. 14. 

 Aug. 14. 

 Aug. 14. 



Aug. 14. 

 Aug. 14. 



Aug. 14. 



Aug. 14. 

 Aug. 14. 

 Aug. 14. 



Aug. 14. 



Aug. 14. 



Aug. 14. 

 Aug. 14. 



Aug. 14. 



Same as above. 



....Do 



...Do 



....Do 



....Do 



Behavior. 



Both thinly clouded. 



Do. 

 Thinly clouded. 



Clear. 

 Both clear. 



Do. 



Both clear. Check 

 tubes of Aug. 14 

 in bouillon have 

 clouded. 

 Both clear. 



Both clear. One 

 cracked on side 

 and fungus has en- 

 tered tiny crack. 



Both clear. 



All clear. 



Do. 

 Both clear. 



Both thinly cloud- 

 ed. Rolling on 

 shaking. 



Thinly clouded. 



Clear. 



Both clouded, the 

 reinoculated one 

 more so. 



Both clear. 



Do. 



Do. 



Do. 



Do. 



Do. 

 Clear. 



Do. 



Do. 



Do. 



Do. 



Do. 

 All clear. 



Conclusion: Bacterium stewarti will grow in this medium but has some difficulty in getting started. Must be inoculated copiously 

 tMediuro evidently not entirely free from nitrogen. 



JComposed of: distilled water 1. 000, dipotassium phosphate 2.0: magnesium sulphate 0.1; ammonium phosphate 0.1; sodium acetate 5- n - 

 September 22: Stock 3415 heavily clouded; stock 3424. doubtful, if any, growth; stock 3427. color much duller than in the check, due 

 to clouding. 



Nitrogen foods. The behavior in Usehinsky's solution indicates ability to use sodium aspara- 

 ginate and ammonium lactate, one or both, under the conditions present. 



The inability to reduce potassium nitrate shows that nitrogen is not obtained from this source. 

 Peptone and the nitrogen in beef-juice are both foods. 



Tests made in 190S with Bact. stewarti from three sources, inoculating copiously, gave the fol- 

 lowing results: 



(1) No growth or doubtful: Asparagin water (2 per cent asparagin); ammonium lactate and cane-sugar in water; 



ammonium citrate and cane-sugar in water; ammonium tartrate and cane-sugar in water. 



(2) Feeble clouding: Asparagin and cane-sugar in water. 



