EXPERIMENT WITH MAN. 179 



FOOD. 



On the experimental day here reported, the diet consisted of 1,652.90 

 grams milk modified by a large proportion of butter fat, and 5 grams 

 of the desiccated milk proteid sold under the trade name Plasmon. In 

 addition to the milk and Plasmon, 139 grams of water were used. 

 These quantities of food furnished 53.31 grams of protein, 21 1.87 grams 

 of fat, 75.41 grams of carbohydrates, and 2,569 calories of energy. 



ROUTINE OF EXPERIMENT. 



The experiment was carried out according to the customary routine 

 established in this laboratory for experiments with the respiration 

 calorimeter. Detailed accounts of this routine have been published 

 elsewhere. 1 Minor, though important, changes in the preparation, sam- 

 pling, and analysis of the milk and cream have since been introduced 

 to facilitate in accuracy and manipulation. 



The feces were separated in the usual way by means of lamp-black 

 capsules, though in experiments either during fasting or immediately 

 following fasting we have experienced great difficulty in securing satis- 

 factory separations. For the want of more satisfactory technique, 

 therefore, we are now in the custom of collecting the total feces for the 

 food period (in this case 3 days), including that passed after the subject 

 has left the respiration chamber, and ascribing an aliquot portion of 

 the feces to each food-day. 



The daily routine followed by the subject in the respiration chamber 

 consisted mainly in rising from bed, dressing, eating, care of food and 

 excreta, sitting at a table, reading or writing, and occasionally stand- 

 ing or taking a few short steps. In general, the subject followed pretty 

 closely a definite program previously prepared. 



Ordinarily the subject enters the respiration chamber at 1 1 p. m. on 

 the day before the actual experiment begins. This enables him to 

 become accustomed to the environment, and affords opportunity to 

 secure constant-temperature conditions inside the chamber after a long 

 night's sleep. In this particular case, the subject had already remained 

 in the chamber for 5 days of fasting, so that there was no preliminary 

 period for the food experiment, which began at 7 a. m. From this time 

 to the close of the experiment a careful record was kept of all data 

 for computing the total income and outgo of matter and energy. So 

 far as the measurements of heat, carbon dioxide, water, and oxygen 

 are concerned, the day was, as usual, divided into 12 periods of 2 hours 

 each. It has not been found feasible to make such short separations 



1 U. S. Dept. of Agr., Office of Experiment Stations Bulls. 44, 63, 69, 109, and 136. 



