606 



STARCHES OF LILIACE^. 



Differentiation of Certain Starches of the Genus Brodioea. Continued. 

 PoLABiscopic Propebties. Continued. Staining Reactions. 



Figure. Continued. 



B. capitala: Eccentric, very distinct, not always clear-cut, 



usually regular. 

 B. congesta: Eccentric, usually distinct, usually clear-cut, 



usually regular. 



Degree of Polarization. 



B. peduncularis: High. 



B. ixoides var. splendens: High, but slightly lower than in 



B. peduncularis. 

 B. Candida: Fairly high, lower than in B. peduncularis. 

 B. lactea: High, slightly higher than in B. peduncularis. 

 B. Una: High, not so liigh as in B. peduncularis. 

 B. coccinea: High, not so high as in B. peduncularis. 

 B. grandiflora: Fairly high, lower than in B. peduncularis. 

 B. califoruica: Fairly high, lower than in B. peduncularis. 

 B. purdtfi: High, about the same as in fi. peduncularis. 

 B. stellaris: High, less than in B. peduncularis. 

 B. capitata: High, slightly higher than in B. peduncularis. 

 B. congesta: Faii'ly high, not so high as in B. peduncularis. 



Polarization with Selentie Quadrants and Colors. 



B. peduncularis: Quadrants not well defined, irregular in 

 shape, unequal in size. Colors pure. 



B. ixoides var. splendens: Quadrants fairly well defined, 

 usually irregular in shape, unequal in size. Colors 

 usually not pure. 



B. Candida: Quadrants fairly well defined, irregular in 

 shape, unequal in size. Colors not quite pure. 



B. lactea: Quadrants well defined, sometimes irregular in 

 shape, unequal in size. Colors pme. 



B.laxa: Quadrants usually well defined, irregular in shape, 

 unequal in size. Colors usually pure. 



B. coccinea: Quadrants fairly well defined, often irregular 

 in shape, unequal in size. Colors usually pure. 



B. grandiflora: Quadrants usually not well defined, irreg- 

 ular in shape, unequal in size. Colors not pure. 



B. californica: Quadrants not well defined, irregular in 

 shape, unequal in size. Colors not pure. 



B. purdyi: Quadrants usually not well defined, generally 

 irregular in shape, unequal in size. Colors fairly 

 pure. 



B. stellaris: Quadrants usually well defined, irregular in 

 shape, unequal in size. Colors usually pure. 



B. capitata: Quadrants well defined, regular in shape, un- 

 equal in size. Colors pure. 



B. congesta: Quadrants well defined, regular or irregular 

 in shape, unequal in size. Colors fairly pure. 



Iodine Reactions. 



Intensity and Color. 



B. peduncularis: Deep; blue-violet. 



B. ixoides var. splendens: Deep, same as in . peduncu- 

 laris; blue-violet. 



B. Candida: Fairly deep, less than in B. peduncularis; blue- 

 violet. 



B. lactea: Deep, deeper than in B. peduncularis; blue- 

 violet. 



B. laxa: Deep, deeper than in B.pedujicutara; blue-violet. 



B. coccinea: Very deep, deeper than in B. peduncularis; 

 blue-violet. 



B. grandiflora: Very deep, deeper than in B. peduncularis; 

 blue- violet. 



B. californica: Deep, same aa in B. peduncularis; blue- 

 violet. 



B. purdyi: Very deep, deeper than in B. peduncularis; 

 blue-violet. 



B. stellaris: Deep, same as in B. peduncularis; blue-violet. 



B. capitata: Very deep, deeper than in B. peduncularis; 

 blue- violet. 



B. congesta: Deep, same as in B.pedttncMfam; blue-violet. 



With Gentian Violet. 



B. peduncularis: Light. 



B. ixoides vax. splendens: Light, same as in B.peduncutoris. 



B. Candida: Fair, deeper than m B. peduncularis. 



B. lactea: Light, deeper than in B. peduncularis. 



B. laxa: Light, deeper than in B. peduncidaris. 



B. coccinea: Fairly deep, deeper than in B. peduncularis. 



B. grandiflora: Light, slightly more than in B. peduncu- 

 laris. 



B. californica: Fairly deep, deeper than in B. peduncu- 

 laris. 



B. purdyi: Fairly deep, much deeper than in B. pedun- 

 cularis. 



B. stellaris: Light, same as in B. peduncularis. 



B. capitata: Fairly deep, much deeper than in B. peduncu- 

 laris. 



B. congesta: Fair, deeper than in B. peduncularis. 



With Safranin. 



B. peduncularis: Light, but deeper than with gentian 

 violet. 



B. ixoides var. splendens: Light, same as in B. peduncu- 

 laris. 



B. Candida: Light, but deeper than in B. peduncularis. 



B. lactea: Light, same as in B. peduncularis. 



B. laxa: Light, same as in B. peduncidaris. 



B. coccinea: Fairly deep, much deeper than in B. peduncu- 

 laris. 



B. grandiflora: Fairly deep, much deeper than in B. pe- 

 duncularis. 



B. californica: Light, the same as in B. peduncularis. 



B. purdyi: Fairly deep, much deeper than in B. peduncu- 

 laris. 



B. stellaris: Light, slightly deeper than in B. peduncularis. 



B. capitata: Fair, deeper than in B. peduncularis. 



B. congesta: Light, slightly deeper than in B. peduncularis. 



Temperature Reaction. 



B. peduncularis: 68 to 69 C, mean 68.5. 



B. ixoides var. splendens: 65.5 to 62 C, mean 6L75. 



B. Candida: 61 to 63 C, mean 62. 



B. lactea: 64 to 65 C, mean 64.5. 



B. laxa: 60 to 62 C, mean 61. 



B. coccinea: 69 to 71 C, mean 70. 



B. grandiflora: 65 to 66.8 C, mean 65.9. 



B. californica: 67 to 68 C, mean 67.5. 



B. purdyi: 66 to 68 C, mean 67. 



B. stellaris: 67.2 to 69 C, mean 68.1. 



B. capitata: 65 to 66 C, mean 65.5. 



B. congesta: 68.5 to 70 C, mean 69.25. 



Effects of Vabiotjs Reagents. 

 Reaction with Chloral Hydrate-Iodine. 



B. peduncularis: Begins in one-third of grains in 4 minutes; 



complete in one-third in 30 minutes. 

 B. ixoides var. splendens: Begins in five-sixths of grains in 



5 minutes^ complete in five-sixths in 25 minutes. 

 B. Candida: Begms in half of grains affected in 5 minutes; 



complete in all in 35 minutes. 

 B. lactea: Begins in all in 5 minutes; complete in all in 45 



minutes. 

 B. laxa: Begins in all in 3 minutes; complete in all in 35 



minutes. 

 B. coccinea: Begins in two-tliirds in 5 minutes; complete 



in all but 2 or 3 in 90 minutes. 

 B. grandiflora: All the small grains, a few of the large 



(equahng about half the total number), in 5 



minutes; complete in nearly three-fourths in 



90 minutes. 

 B. californica: Begins in one-third in 5 minutes; com- 

 plete in one-third in 60 minutes. 



