GENUS ANEMONE. 



855 



Curve of Reaction-Intensities ' 



fulgens. 



A Starch of 



MINUTES 

 Anemone 



The degree of polarization is low in most grains, but is fairly high in some of the larger ones. 

 It does not vary much in different aspects of the same grain. It is slightly higher than in the grains 

 of A . apennina. 



_ With seknile the quadrants are, as a rule, fairly well defined, irregular in shape, and unequal 

 in size. In the smaller grains the colors are pm-e, not in the larger. 



Iodine Rcadionii.- -V^"\i\\ 0.2.5 per cent LugoFs solution the grains arc colored at once deeply, 

 and the color deepens very rapidly until the grams are almost hiack; the depth is the same as that 

 of the grains of A. apennina. With 0.125 per cent solution the grains color at once and the color 

 deepens rapidly, but the shade is not so deep as that of 

 the grains of A. apennina. After heating in water until 

 all of the grains are gelatinized, the solution is colored 

 very lightly and the grains very deeply on the addition 

 of iodine. After boiling for 2 minutes the solution colors 

 much more deeplj', the grain-residues much less or not 

 at all. On the addition of an excess of iodine the grains 

 show a red-violet capsule. No grains are completely 

 disintegrated. 



Staining Reactions. With gentian violet the grains 

 begin to stain at once and in 30 minutes they are fairly 

 stained. The color is lighter than that of the grains of 

 A. apennina. 



With safranin the grains begin to stain lightly at 

 once, but after 30 minutes the color is light, slightly 

 lighter than that of the grains of .4. apennina. 



Temperature Reaction. The temperature of gelatin- 

 ization is 50 to 51.5 C, mean 50.75. 



Effects of Various Reagents. AVith chloral hydrate- 

 iodine reaction begins at once and is over in 13^ minutes. 

 The reaction is identical qualitatively but not quantitatively with that of the grains of A. apennina. 

 These grains do not react quite so rapidly, hence the process is more easily followed than in the 

 grains of A. apennina. 



The reaction with chromic acid begins at once and is over in 30 minutes. This reaction is quali- 

 tatively identical with that of A . apennina. 



The reaction with pyrogalHc acid begins at once and is over in 2 minutes. Qualitatively it is 

 the same as that of the grains of A. apennina. 



Reaction with ferric chloride begins in 20 seconds and is over in 3 minutes. Qualitatively it 

 is the same as that of the grains of A . apennina. 



Reaction with Purdy's solution begins at once and is over in a very few seconds. It is impossible 

 to determine the steps of the reaction. 



STARCH OF ANEMONE BLANDA. (Plate 94, fig. 564. Chart 371.) 



Histological Characteristics. In forin the grains are simple. There are no aggregates, very 

 rarely pressure facets, and there is some tendency to clumps which are easily broken apart. The 

 surface of the grains shows some irregularities due to the irregular development of surface, chiefly 

 in the form of nipple-like processes. The conspicuous forms are ovoid, elliptical, oval, lenticular, 

 triangular. There are round or nearly round, spindle-shaped, and various indefinite forms. The 

 grains are sometimes bent at or near the middle; they are not flattened in any diameter, but may 

 vary in form according to the aspect viewed, owing to irregular development. 



The hilum is not very distinct. A depression on the surface of the grain is always present at 

 this point. In some forms it is centric. In some of the shorter forms the hilum is a round spot, 

 eccentric about one-third of the longitudinal axis and usually in the median line. In the longer 

 forms it is elongated or lenticular. When the grains are subjected to chemical reagents the elon- 

 gated hilum is often seen to be a row of 2 or more hila. The hilum itself is not fissured. 



The lamellce are invisible. 



The grains vary in size from 2 to IQ/x. The dimensions of the lenticular or triangular grains 

 are 10 by 6/i in breadth and length. The common size is 10/u. 

 56 



