MEDIA. 



49 



Egg-yolk. This is poured into test-tubes and solidified in a slanting position 

 by heat (80 C.), or the egg may be boiled hard and the yolk cut with a sharp 

 knife and transferred to sterile Petri dishes. If desired, the yolk and white may 

 be mixed before solidifying, /. e., by shaking the egg vigorously before breaking 

 the shell. 



SYNTHETIC MEDIA AND OTHER SPECIAL MEDIA. 



The student should try the following media. He should also invent media to 

 suit special cases. The kinds of media I have in mind are the opposite of universal, 



Fig. 45.* 



to-wit, such as will favor the growth of some organisms while preventing that of 

 others. The acid phosphates and many other substances are useful for this purpose. 

 The field is comparatively new, and much is to be learned by careful experimenting. 



1. Dunham's solution. 



2. Peptone-water (i or 2 per cent) with addition of various carbohydrates, 

 acids, etc. 



3. Sugar-free beef bouillon with Witte's peptonuin siccutu (for the indol test). 



4. Cohn's solution. 



5. Uschinsky's solution. 



*Fig. 45. Oven for solidifying and sterilizing blood-serum, nutrient starch-jelly, silicate-jelly, 

 etc., at temperatures below 100 C. When in use the temperature <is controlled by means of a 

 Tollen's thermo-reg-ulator (see fig. 35). 



