HiRMUL.F.. 



197 



SYNTHETIC CULTURE MEDIA. 



I'aslctti j s Culture /'liiiil. 



Ammonuiin tarlrate 10 



Ashes iif vast 10 



Rock candy 100 



Distilled water 1,000 



1 >isso]\ e cold. 



Naegeli's Nutrient Solution. 



Calcium chloride O.I 



Magnesium sulphate .2 



Dipotassium phosphate.. i.o 



Ammonium tartrate 10.0 



Distilled water 1,000.0 



( \>lin's .\'nti'ic:il Solution. 



Distilled water 1,000.0 



Acid potassium phosphate 5.0 



Magnesium sulphate 5.0 



Neutral ammonium tartrate IO.O 



Potassium chloride 0.5 



(De Bary, p. 86, Vorles. ii. Buet, 2 Auflage.) 

 Ra itlin's Culture-Flu id. 



Distilled water 1,500.00 



Granulated cane sugar 70.00 



Tartaric acid 4.00 



Ammonium nitrate 4.00 



Ammonium phosphate .60 



Potassium carbonate .60 



Magnesium carbonate .40 



Ammonium sulphate .25 



Zinc sulphate .07 



Ferrous sulphate .07 



Potassium silicate .07 



Prazmowski's Culture-Fluid. 



Dipotassium phosphate 5.0 



Magnesium sulphate 5.0 



Ammonium carbonate 5-O 



Calcium chloride -5 



Distilled water 1,000.0 



Dissolve cold. Any desired sugar may be 

 added for the carbon food. 



.!<l, 'If Mnycr's Ciiltiiri'-I'lunl. 

 (Unters. ii. d. ale. Gahr., 1870.) 



Magnesium sulphate 10.0 



\tmnonium nitrate 15- 



Tri-basic calcium phnsphat'- - 1 



Potassium phosphate 10.0 



Distilled water 1,000.0 



Dissolve cold and add sugar. Add sodium 

 chloride (3 per cent) if it is to be used for 

 luminous bacteria, and an excess of pure ear- 

 In mate of lime if acid-forming bacteria are to be 

 grown. 



Uschinsky's St>lnti>n. 



Distilled water 1,000 



Glycerin 30 to 40 



Sodium chloride 5 to 7 



Calcium chloride o.i 



Magnesium sulphate 0.3100.4 



Dipotassium phosphate 2 to 2.5 



Ammonium lactate 6 to 7 



Sodium asparaginate 3 to 4 



Mudifici/ Uschinsky's Sulniinn. 

 The modified Uscliinsky recommended by the 

 writer for use with starch jelly is made as fol- 

 lows : 



Distilled water I.OOO.OOO 



Ammonium lactate 5.000 



Sodium asparaginate 2.500 



Sodium sulphate 2.500 



Sodium chloride 2.500 



Dipotassium phosphate 2.500 



Calcium chloride .010 



Magnesium sulphate .010 



Fraenkel tunl fuses' S iln/imi. 

 (Hygienische Rundschau, Bd. IV, 1894, p. 769.) 



Water i.ooo 



Sodium chloride 5 



Dipotassium phosphate* 2 



Ammonium lactate 6 



Sodium asparaginate 4 



This paper also discusses I'sdiinsky's solution. 

 Fermi's Culture-Fluid. 



Distilled water i.ooo.o 



Magnesium sulphate .2 



Acid potassium phosphate I.o 



Ammonium phosphate IO.O 



Glycerin 45.0 



This may be added to agar in place of pepton- 

 ized beef-broth (De Schweinitz) or to silicate 

 jelly, in which case the volume of water must 

 be reduced (see Silicate Jelly, p. 36). 



Mimre's Culture-Medium fur Leguminous Root- 



tnhcrclc I-iiicilli. 



(For field use.) 



The dried culture (on cotton) is thrown into 

 clean water containing : Cane-sugar, I ; c. p. 

 monopotassium phosphate', o.i ; c. p. magnesium 

 sulphate, o.oi per cent. After 24 hours add 

 Merck's pure dibasic ammonium phosphate to 

 amount of 0.5 per cent. Seeds are drenched 

 with this fluid at end of another day, dried in 

 shade, and planted. 



*I,e!imanu aud Neumann recommend neutral commercial sodium phosphate ('. c. p. 29.) 



