CAPSULES; STAINS AND STAINING METHODS. 



221 



('92). LIESENBERG, C., u. ZOPF, W. Ueber den sogen- 

 annten Froschlaichpilz (Leuconostoc) der 

 europaischen Riibenzucker- und der javan- 

 ischeu Rohrzuckerfabriken. Zopf's Beitraege, 

 Hit. i, 1892, pp. 1-29, with 2 plates, and 

 Hft. ii, 1892, pp. 1-2. Rev. in Am. Nat., 

 March, 1897, p. 228. 



('92). WKLCH, WM. H. A gas-producing bacillus 

 (B. aerogenes capsulatus, nov. spec.) capable 

 of rapid development >in the blood-vessels 

 after death. Bull. Johns Hopkins Hospital, 

 vol. 3, No. 24, July-August, 1892, pp. 81-91. 

 Also a .separate. 



('94). KOCH, ALFRED, AND HOSAEUS, HANS. Ueber 

 einen neuen Froschlaich der Zuckerfabriken. 

 lYntralb. f. Bakt, Bd. xvi, 1894, pp. 225- 

 228, mit i fig. 



Describes a branching gelatinous organism as Bact. 

 pediculatum. The branching is due to a terminal split- 

 ting or one-sided development of the capsule. 



(*9S). STIFT, A. Ueber die in den Produkten der 

 Zuckerfabrikation auftretenden Bakterien. 

 Centralb. f. Bakt, 2 Abt., Bd. I, 1895, pp. 

 277-283. 



('96). WILDE, MAX. Ueber den Bacillus pneumonias 

 Friedliinder's und verwandte Bakterien. 

 Inaugural Dissertation, Bonn, 1896, pp. 74. 

 Bibliography of 89 titles. Printed by Carl 

 Georgi. 



('96). FRICKE, CARL. Ueber den sogenannten Bacil- 

 lus mucosus capsulatus. Zeitschr. f. Hyg., 

 Bd. xxni, 1896, pp. 380-451, with a bibliog- 

 raphy of 25 titles. 



('98). PANE, N. Ueber die Genesis der Kapseln des 

 Pneumococcus. Centralb. f. Bakt., xxiv Bd., 

 1898, pp. 289-294, with figs. 



The capsule is the swollen outer part of the bacterium. 



('98). KAI.I'.MANN. Eine neue Methode zur Farbung 

 von Bakterienkapseln. Hyg. Rundschau, 



1898, No. 18. Abstr. Centralb. f. Bakt., I 

 Abt., Bd. xxv, p. 32. 



('99). WARD. See XLVII. 



('99). MOORE, A. Capsule staining. Trans. Jenner 

 Inst., 2(1 sen, pp. 244, 1899. 



Recommends a contrast stain for the capsule. The 

 preparation is first fixed with dilute acetic acid, then 

 stained in carbol fuchsin for about i minute, washed in 

 distilled water and dried. It is then stained, with or 

 without gentle heat, in night blue for i or 2 minutes, 

 washed and dried. The night blue solution is Morton's 

 modification of McCrorie's stain. (See Morton, Flagella 

 staining. ) By this method the capsule is stained blue and 

 the body dark red. 



('99). STRONG, LAWRENCE WATSON. Ueber die Kap- 

 selbacillen. Centralb. f. Bakt., xxv Bd., 



1899, pp. 49-52. 



('oo). BONI, ICILIO. Methode zur Darstellung einer 

 " Kapsel " bei alien Baktcrienarten. Centralb. 

 f. Bakt., xxvni Bd., 1900, pp. 705-707. 



('01). WARD. See XLVII. 



foi). BONI, I. Ricerche sulla capsula dei batteri. 

 Giorn. Soc. ital. igiene, Milano, vol xxni, 

 1901, pp. 417-430. 



('02). SMITH, R. GREIG. An Ascobacterium from 

 the sugar-cane, with notes upon the nature 

 of the slime (Bacterium sacohari, n. sp.). 

 I': Breedings of Linnean Soc. of New South 

 \\ riles, vol. xxvii, 1902, part i, pp. 137-14^. 

 i plate. Also a separate (issued Aug. 22, 

 1902). See also Central!), f. Bakt, 2 A'bt 

 Bd. ix, p. 806. 



XIV. Stains and Staining Methods. 

 (See also XI, XII, and XIII.) 



EHRI.ICH. Beitrage zur Kenntniss der Anilin- 

 farbungen und ihrer Verwendung in der 



mikroskopischen Technik. Arch. f. mikr. 



Anat. Bd. xm, p. 263. 

 ('75). WEIGERT. Farbung von Bakterien. Ber. iiher 



d. Sitzungen d. sehlesisch.cn Gesellsch. f. 



vatenl. Cultur, 10 Dec., 1875 

 ('77). KOCH. See LV. 

 ('81). WEIGERT. Zur teohnik der mikroskop. Bak- 



terienuratersuchungen. Virdi. Archiv. Bd. 



LXXXIV, iSSi, p. 275. 

 ('82). EHRLICH. Farlbung der Tuberkelbacillan 



Verhdlgn. d. Ver. f. i. Med., i Mai, 1882. 



D. mecl. Woch., 1882, p. 269. 

 ('82). ZIEHL. Zur Farbung des Tuberkelbacillus. 



Deutsche med. Wochensc.hr., 1882, No. 33 



P- 451- 

 ('82). SZYSZYLOWICZ, J. Das Corallin als mikro- 



chenusches Reagens in d. Pflanzenhistologie, 



R. i. S. Ak. Krakau, T. x, 1883, pp. 97-114. 



Not seen. Rev. Bot. Centralb., 1886, Bd 



xxvin, p. 51. See also Bot. Centralb., li.l 



xn, 1882, p. 139. 



This substance should be dissolved in sodium carbon- 

 ate. It is iised as a test for slime derived from starch 

 which stains diffusely purple or rose. After staining the 

 preparation should be subjected to hot alcohol, which 

 removes the stain from all but the starch grains and 

 slime derived therefrom. Gum slitne and cellulose slime 

 are bleached, the former even in cold alcohol. Pure gum 

 does not stain. Mount the preparation in Canada balsam. 



('83). ZIEHL. Zur Lehre von den Tuberkelbacillen, 

 insbesondere iiber deren Bedeutung fur 

 Diagnose und Prognose. Deutsche med. 

 Woohenschr., 1883, p. 62. 



('84). KOCH. See vi. 



I 'N \ ) . LOEFFLER. See VI. 



('84). GRAM, C. Ueber die isolirte Farbung der 

 Schizomyceten in Schnitt-und Trocken- 

 praparaten. Fortschr. d. Med, Bd n No 

 6, 1884, pp. 185-189. 



('86). PFEFFER, W. Ueber Aufnahme von Aniliu- 

 farben in lebende Zellen. Untersuchungen 

 a. d. hot. Inst. Tubingen, n, p. 179, 1886. 



('87). UNNA. Die RosaniJine und Pararosaniline, 

 eine bacteriologische Farbenstudie. Derma- 

 tologischen Studien. Heft 4, pp. 9-73. 

 _ Hamburg, Voss, 1887. 



('87). KUEHNE. Ueber ein combinirtes Universal- 

 verfahren, Spaltpilze im thierischen Gewebe 

 naohzuweisen. Dermatol. Studien, heraus- 

 geg. von Unna. Heft 6. Hamburg, L. Voss, 

 1887. 



('87). HEIM, L. Die Neuerungen auf dem Gebiete 

 der bakteriologischen Untersuchungsmeth- 

 oden seit dem Jahre, 1887, I. Farbunsjs- 

 methoden. Centralb. f. Bakt., x Bd., 1891, 

 pp. 260-265, 288-296, and 323-328. 

 ('88). KUEHNE, H. Praktische Anleitungzum mikro- 

 skopischen Naohweis der Bakterien im 

 thierisohen Gewebe. Leipzig (Giinthers), 

 1888, pp. 15-23, Methylenblaumethode 

 ('88). UNNA, P. G. Die Entwicklung der Bak- 

 terien farbung. Eine historisch-kritische 

 Uebersicht. Centralb. f. Bakt., 1888, in Bd., 

 pp. 22-26 ; also pp. 61-63, 93-99, 120-125, IS3- 



158, t89-I9S, 2I8-22T. 254-2^9, 285-291, 312- 

 320, 345-31^. 



At the end 71 paper* on this subject are mentioned by 

 title. 



