222 



BACTERIA IN RELATION TO PLANT DISEASES. 



('91 ). PREGL, FRITZ. Ueber eine neue Karbolmethy- 

 lenblau-Method. Ceatralb. f. Bakt., x Bd., 

 1891, pp. 826-829. 



Pregl finds the following method of staining sections 

 on slides satisfactory : " Exposure for one-half to one 

 minute to carbol methylen blue, sometimes with the aid 

 of heat; second, brief washing in water; third, bleaching 

 in 50 per cent alcohol until the section has become pale 

 blue passing into greenish ; fourth, removal of the water 

 by means of absolute alcohol ; fifth, clearing in xylol ; 

 sixth, enclosure in balsam." 



Kuehne's carbol methylen blue is made as follows : 1.5 

 grins, methylen blue, 10 grins, absolute alcohol, loogramss 

 percent carbolic-acid water. The alcohol is poured over 

 the mtrthylcu blue ; the carbolated water is then added and 

 rubbed up thoroughly with it. If only a small quantity 

 is needed from time to time, it is better to make it up in 

 less amounts, as, with time, the staining power of the 

 solution decreases. 



('92). KuEHNE, H. Das Malachitgriin als Auszieh- 

 ungsfarbe. Centralb. f. Bakt., xi Bd., 1892, 

 PP. 7S6-7S8. 



Author finds malachit green dissolved in anilin oil very 

 useful for treatment of sections stained in carbol-fuch- 

 siu, etc. The bacteria stand out distinctly deep red on a 

 bluish background. See paper for details. 



('93)- NICOLLE, ET CANTACUZENE, J. Proprieties 

 colorantes de I'oxychlorure de ruthenium 

 ammoiuacal. Ann. de 1'Inst. Pasteur, T. 

 vii, pp. 331-334- 



('9S). UNNA, P. G. Ueber Verwendung von Anilin- 

 mischungen zur tinktoriellen Isolierung von 

 Gewebselementen. Monatshefte f. prakt. 

 Dermatologie, Bd. xxi, 1895. Rev. in Cen- 

 tralib. f. Bakt., I Abt., xx Bd., 1896, p. 406. 



('95)- NICOLLE, M. Pratique des colorations micro- 

 biennes, Methode de Gram modifiee et 

 methode directe. Ann. de 1'Inst. Pasteur, 

 T. ix, 1895, No. 9. Rev. in Centralb. f. 

 Bakt., XVHI Bd., 1895, p. 552. 



Carbol violet is substituted for nniliu violet. Excess of 

 color is removed withalcohol-aceton. 



('<j?). SKMENOWICZ, W., UND MARZINOWSKY, E. 

 Ueber ein besonderes Verfahren zur Far- 

 bung der Bakterien im Deckglaspraparate 

 und in Schnitten. Centralb. f. Bakt., xxi 

 Bd., 1897, pp. 874-876. 



Coo). DREYER, GEORGES. Bakterien farbung in gleich- 

 zeitig naoh van Gieson's Methode behandel- 

 ten Schnitten. Centralb. f. Bakt., I Abit., 

 xxvn Bd., 1900, pp. 534-535- 



Can be used for differential staining of animal tissues 

 with bacteria in them, provided the latter are such as 

 stain by Gram's method. 



Coo). LE Doux. Bemerkungen zti dem Artikel des 

 Herrn M. Dorset: "A new stain for Bacillus 

 .tuberculosis." Centralb. f. Bakt., I Abt., 

 xxvn Bd., 1900, p. 616. 



Dorsett recommended Sudan III for staining the B. 

 tuberculosis. Le Doux says he has tried this repeatedly 

 \\ ilh entirely negative results. 



('02). LEVINSON, JA. B. Ueber Farbung des Fettes 

 und der fettigen Degeneration der geform- 

 len Elemente in fiiissigen und halbfliissigen 

 Medien mit Sudan in. Wratsch. St. 

 Petersberg, 1902, Bd. I. pp. 1,208-1,209. 

 (Russian.) 



''.RIMME, ARNOI.II. Die wiclitigsten Methoden 

 der Bakterienflirbung in ihrer Wirkung auf 

 die Membran, den Protoplasten und die 

 Einsohliisse der Bakterienzelle. Centralb. f. 

 Bakt., Abt. I, Bd. xxxn, Originale, 1902, pp. 

 1-16, pp. 81-90, pp. 161-180, pp. 241-255, pp. 

 321-327, with 2 plates. Bibliog. of 78 titles. 



XV. Morphological and Physiological Changes Due 

 to Changed Environment. 



('87). SCHOTTELIUS. Untersuchungen uber den 

 Micrqc. prodigiosus. Festschr. f. Kdlliker, 

 Leipzig, 1887. Not seen. 



This author obtained pigmeuted and non-pigmented 

 races of Bacillus prodigiosus. 



('89). BEHRING. Beitrage zur Aetiologie des Milz- 



brandes. vi. Ueber asporogenen Milzbrand. 



Zeitschr. f. Hyg., 1889, Bd. vn, pp. 171-176. 



Author describes method of obtaining Aplanobacter 



(Bacillus) authracis without spores. 



('90). SMITH. See vi. 



('90). Roux, E. Badteridie charbonneuse asporogene. 

 Ann. de 1'Inst. Pasteur, T. iv, 1890, pp 25-34 



('92). NENCKI, M. Ueber Mischkulturen. Centralb. 

 f. Bakt., xi Bd., 1892, pp. 225-228. 



('92). ADAMI, J. G. On the variability of bacteria 

 and the development of races. Med. Chron- 

 icle, vol. xvi, 1892, No. 6, pp. 366-389. Also 

 a reprint from Med. Chronicle, Sept., 1892, 

 Svo., 26 pp., Manchester (John Heywood), 

 1892. 



('92). CHARRIN, A., ET PHISALIX. Abolition nersis- 

 tante de la fonction chromogene du Bacillus 

 pyocyaneus. C. R. des se. de 1'Acad. des 

 Sci., Paris, 1892. T. cxiv, pp. 1,565-1,568. 



('93)- SANDER. Ueber das Wachstum von Tuber- 

 kelbacillen auf pflanzlichen Nahrboden 

 Arch. f. Hyg., Bd. xvi. Heft 3, 1893, pp. 

 238-311. 



At 38- 39 C. (not at 22-23 C.), this author induced the 

 organism of tuberculosis to grow on a variety of vegeta- 

 ble substances, i, e., potato, carrot, kohlrabi macaroni 

 etc. All were cooked. 



('93). STONEY, G. Suggestion ias to a possible source 

 of the energy required for the life of bacilli, 

 and as to the cause of their small size. 

 Proc. Roy. Soc., Dublin. Vol. vni, Pt. I, 

 pp. 154-156- Dublin, 1893. 

 Views of a physicist. 



('94). SURMONT, H., ET ARNOULD, E. Rccherohes 

 sur la production .du bacille du charbon 

 asporogene. Ann. de 1'Inst. Pasteur, T. vni 

 1X94, PP- 817-832. 



('94). URY, JAKOB. Ueber die Sohwankungen des 

 Bacterium ooli commune in morphologis- 

 cher und kultureller Beziehung. (Inaug. 

 Diss.) Svo., 47 pp., Strassburg i. E., 1894. 

 Rev. in Centralb. f. Bakt., Bd. xvi, 1894, pp. 

 579-581. 



('94). SMITH, THEOBALD. Modification, temporary 

 and permanent, of the physiological char- 

 acters of bacteria in mixed cultures. T.rans. 

 Assoc. Amc-r. Physicians, Phila., 1894, pp 

 85-109. 



('94). DIEUDONNE, A. Beitrage zur Kenntnis der 

 Anpassungsfahigkeit der Bakterien an ur- 

 sprunglich ungunstige Temperaturverh;ilt- 

 nisse. Arbeiten aus dem kaisenl. Gesund- 

 heitsamte, Bd. ix, 1894, pp. 492-508, Berlin, 

 1894 CJulius Springer). Also a separate. 

 Rev. in Cenitralb. f. Bakt., Bd. xvi, 1894, pp. 

 '1115-967. 



('95). DAVENPORT, C. B., AND CASTLE, W. E. On the 

 acclimatization of organisms to high tem- 

 peratures. Archiv. f. Entwiokelungsme- 

 ahanik der Organismen, Bd. ir. Heft 2, 1895, 

 pp. 227-240. Bibliography of 3 pages. 



Organisms become acclimatized to high temperature by 

 losing water from their protoplasm. 



