METHODS OF \V<)U|<; MKANS OF DIFFERENTIATING. 



229 



('02). CARNOT, PAUL, ET GARNIER, MARCUL. Sur la 



technique des cultures en tubes de sable. 



Paris, C. R. soc. biol., T. LIV, 1902, pp. 748- 



750. 

 ('02). CARNOT, PAUL, ET GARNIER, MARCEL. De 



IVmploi des tubes de sable comme inethode 



gOnerale d'etude, d'isolement et de selection 



des microorganismes mobiles. Paris, C. R. 



soc. biol., T. LIV, 1902, pp. 860-863. 

 ('02). RECAUD, CL. Nouveau bain de paraffine a 



cbauffage et regulation electriques. J. anat. 



physiol., Paris, T. xxxvm, 1902, pp. 193- 



-M4, av. fig. 



('02). GRIJNS, G. Eine einfache Vorrichtung, inn 

 zu verliindern, dass beim Gebrauch des 

 Briitapparates fiir konstante niedrige Tem- 

 pcratur, System Lautenschlager (Katalog 

 No. 60, No. 117), wenn das Eis im Behalter 

 ausgeht, das ungekiiblte Wasser in den 

 kalten Schrank fliesst. Centralb. I. Bakt., 

 Abt. I, Bd. xxxi, Originate, 1902, pp. 430-432. 



('02). HARRIS, NORMAN MACLEOD. Concerning an 

 improved method of making collodium sacs 

 Centralb. f. Bakt., Abt. I, Bd. xxxn, Orig- 

 inale, 1902. pp. 74-80. 

 Dr. Harris makes his sacs around gelalin capsules. 



('03). GORSLINE, CHARLES S. On the preparation 

 and use of collodium sacs. Vaughan Quarter 

 Century Book, 1903, pp. 390-394. Ann Arbor, 

 Mich., George Wahr. 



('03). WIN SLOW, C. E. A., AND NIBECKER, C. P. The 

 significance of bacteriological .methods in 

 sanitary water analysis. Technology Quar- 

 terly, vol. xvi, 1903, pp. 227-239. Also a 

 separate. 



('04). REMLINGER, P. Le passage du virus rabique 



a travers les filtres. 2e mem. Ann. de 1'Inst. 



Pasteur, T. xvin, 1904, pp. 150-164. 

 ('04). Report of the [English] committee appointed 



to consider the standardisation of methods 



for the bacterioscopic examination of water. 



Journal of State Medicine, August, 1904. 



See also Chemical News, vol. xc, Oct. 7, 



1904, pp. I/7-I79- 



Distilled-water agar and distilled-water gelatin are 

 recommended for use along with nutrient agar and 

 gelatin. In the search for B. colt the committee recom- 

 mends either the glucose-formate broth of Pakes or the 

 bile-salt broth of MacCoukev. 



XVIII. Special Means of Differentiating Bacteria. 



('84). GRAM. See xiv. 



('87). VON ROZSAHEGYI, A. Ueber das Zuchten von 

 Bakterien in gefarbter Nahrgelatinc. Cen- 

 tralb. f. Bakt., n Bd., 1887, No. 14, pp. 418-424. 



Author added various substances to his culture media 

 e. g. fuchsin, methylen blue, gentian violet, vesuvin, etc. 

 Different bacteria behave very differently as respects 

 growth, absorption of pigment, and change of color in 

 the pigment. Organisms may be differentiated in this 

 way. 



('88). BU.TWID, O. Neue Methode zum Diagnos- 

 ticiren und Isoliren der Cholerabakterien 

 Centralb. f. Bakt., 1888, iv Bd., pp. 494-496. 

 _ In 24 hours, in 2 per cent feebly alkaline peptone solu- 

 tion at 37" C., the cholera organism gives a fine purple-red 

 color on adding HC1. On longer cultivation other organ- 

 isms give the same reaction. This depends on the forma- 

 tion of iudol and a trace of nitrite. 



('90). PETRUSCHKY, JOHANNES. Die Farbenreaktion 

 bakterieller Stoffwechselprodukte auf Lack- 

 mus als Beitrag zur Charakteris.tik und als 

 Mittel zur Unterscheidung von Bakterien- 

 arten. Centralb. f. Bakt., vn Bd., 1890, pp. 

 1-8 and 49-53- 



('92). BUJWID, ODO. Eine neue biologische Reaktion 

 fiir die Cholerabakterien. Centralb. f. Bakt., 

 xn Bd., 1892, pp. 595-596. 



('92). BEYERINCK, M. W. Notiz fiber die Cholera- 

 roMireaktion. Centralb. f. Bakt., xn Bd 

 1892, pp. 715-718. 



('93). SCHILD. Formalin zur Diagnose des Typhus- 

 bacillus. Centralb. f. Bakt., Bd. xiv~ 1893, 

 PP. 7I7-7I8. 



The typhoid organism will not grow in bouillon con- 

 taining as little formalin as 1:15,000. B. coli develops 

 vigorously with as much formalin as 1:3000. 



('93). MATHEWS, ALBERT P. On Wurtz's Method 

 for the Differentiation of Bacillus typhi 

 abdominalis from Bacillus coli corn-munis, 

 and its application to the examination of 

 contaminated drinking water. Technology 

 Quarterly, vol. vi, 1893, pp. 241-251. 



Litmus lactose gelatin or agar is reddened by B. coli 

 and is unchanged or made deeper blue by B. typhosus. 

 Author tests suspicious water by poured plates. After 14 

 hours at 37.5 C. all blue colonies resembling typhoid are 

 tested further, i. e., in milk, gelatin, bouillon, nitrate 

 solution, and sugar media. 



('93)- GORINI, KONSTANTIN. Anmerkung iiber die 

 Cholerarotreaktion. Centralb. f. Bakt., Bd. 

 xni, 1893, pp. 790-792. 



A good peptone for this purpose must be white, without 

 odor, entirely soluble in water, especially on warming 

 The water solution must be clear, colorless, neutral or 

 slightly alkaline, foamy on shaking. It must give a vio- 

 let reaction with Fehling's solution, which does not 

 change on boiling. It must give no nitrite reaction with 

 Gnesz reagent, and finally must give, after about 5 min- 

 utes with Diphenylamine, a faint but distinct narrow 

 clear blue ring. 



('94)- SCHNEIDER, PAUL. Die Bedeutung der Bak- 

 terienfarbstoffe fiir die Unterscheidung der 

 Arten. (Inaug. Diss.) 8vo., 46 pp., 2 Taf., 

 Basel, 1894. Rev. in Centralb. f. Bakt., Bd. 

 xvi, 1894, p. 633. See also Arbeiten a. d. 

 bakt. Institut Karlsruhe I, 1894. 



('94). MARPMANN. Zur Unterscheidung des Bacillus 

 typhi abdominaliis vom Bacillus coli com- 

 mune. Centralb. f. Bakt., Bd. xvi, 1894, pp 

 817-820. 



Uses various " chromo-agars " for differentiating bac- 

 teria, i.e., agar with fuchsin, malachit green, nigrosiu 

 and indohn. Sodium bisulphit must be used with tin- 

 the first two. 



('94). LUNKEWICZ, M. Eine Karbenreaktion auf die 

 salpetrigc Saure der Kultnren der Cholera- 

 bacillen und einiger anderer Bakterien 

 Centralb. f. Bakt., Bd. xvi, 1894, PP- 945-949. 



Describes the preparations of the Griess-IIosvav re- 

 agent. 



('94). ABEL, RUDOLF. Ueber die Brauchbarkeit der 

 von Sohild angegebenen Formal inprobe zur 

 Differential-Diagnose des Typhus bacillus 

 Centralb. f. Bakt., Bd. xvi, 1894, pp. i 041- 

 1,046. 



Finds some bacteria more tolerant of formalin than 

 was stated by Schild. It is not a satisfactory method for 

 separating typhoid bacilli irom B. coli. 



