INDEX. 



Abbe camera 130 



Abstracts, making of 1 14 



Acclimatization to liig-h temperatures 222 



Acetic alcohol. 



as a fixing fluid 202 



with mercuric chloride 202 



Acetone, for use in fermentation-tubes 52 



Acid-fast bacteria iSS 



Acid-forming bacteria, demonstration by plate 



cultures 51,233 



Acid gelatin, effect on growth 30 



Acid, 



hydrochloric, in culture-media 98 



oxalic, actively germicidal 249 



Acids, 



action on enzymes 68 



effect on bacteria 249 



formation of, in milk-cultures 46 



method of estimating in planls, when not 



free 207 



spilled, neutralization of 107 



toleration of, by bacteria 70, 249 



vegetable, sensitiveness of bacteria to 69 



Achromatium 162 



Acrolein, germicidal action of 252 



Achromatic objectives, 



disadvantages of 140 



with excellent definition 265 



Aerobe, Pasteur's definition of 230 



Agar-agar, 



combined with bouillon and sugars 33, 5 1 



commercial, source of 31, 225 



composition of 32, 223 



cultures on, for flagvlla staining 20 



filtration of 33, 224, 225 



glycerinated 50, 196 



litmus lactose 196 



melting point of 32 



methods of making 223, 224, 225 



nutrient, centrifuging of 225 



nutrient, temperature at which poured 105 



per cent used for media 35 



rate of diffusion in 224 



roll-cultures of 36 



softening of, by bacteria 32 



solidification of 32 



source of 31 



standard nutrient, preparation of 33. "J5 



sterilization of 98, 99 



variation on, of same organism 180 



with neutral red 230 



without nutrients 229 



Page. 



Agar-block method for study of antagonism. . 73 

 Agar-.plates, for detection of acid-forming 



colonies q; I 



Agfa-intensifier 142 



Air, 



bacteria infrequent in Arctic 255 



bacteria rare in upper strata of 255 



of mountains, bacteria rare in 255 



over the sen. bacteria rare in 255 



Air-currents, danger of 103, 106 



Air-shaft, for ventilation of dark-room 149 



Albumen, egg, 



Mayer's 119 



preparation of. for culture-medium 48 



Alcohol, 



as a fixing fluid 8, 202 



ethyl, for use in fermentation-tubes 52 



methyl, for use in fermentation-tubes 52 



weak germicidal action of 252 



Alkalies 



action on enzymes 68 



formation of, in cultures 61 



sensitiveness of bacteria to 69 



toleration of, by bacteria 70 



Alkaline metbylene blue 188 



Amaryllis, inoculated with B. hyacinth! 66 



Amido-bacteria 175 



Amins, test for 64 



Ammonia-bacteria 175 



Ammonia, 



oxidation of, by bacteria 64 



test for 64 



Ammonium, 



citrate, relation to pigment-production 65 



lactate, relation to pigment-production 65 



oxalate, solvent of middle lamella 67 



salts, oxidation of, by bacteria 64 



salts, sterilization without loss of ammonia. 52 



succinate, relation to pigment-production. . . 65 



Amoebobacter 163 



Anaerobe, Pasteur's definition of 230 



Anaerobes 51 



Arens' method of cultivating : 231 



Buchner's method for growing 231 



Bulloch's method of obtaining 228 



Heim's method 231 



Hesse's method 230 



Klein's apparatus for 232 



on culture-media in hydrogen, etc 57 



present in milk 46 



Wright's methods of cultivating 228 



269 



