NEW OBSERVATIONS ON OVARIAN GRAFTING. 13 



The three cases in which young' were born are as follows: 



Guinea-pig- No. 77, blue, age five months, underwent a double ovarian 

 operation in which grafts were taken from a pale cinnamon agouti guinea- 

 pig, age one week. Both grafts were placed on the right side at the uterine 

 horn. Six months later she bore by a white male, No. 654 (fig. 3, pi. l), 

 a blue female young one . She was killed eight months after the operation 

 and ovarian tissue was demonstrated at the right ovarian site, while the 

 graft had completely disappeared. 



Guinea-pig No 24, an albino, age four weeks, was grafted from a black 

 animal one day old. One graft was stitched to the body- wall on each side. 

 Two and a half months later she was noted p regnant, and at three months 

 she had, by male 1078 (a brown-eyed yellow), two young, both black-eyed 

 yellow. Her post mortem two days later showed indications of ovarian 

 tissue at both ovarian sites, but the microscopic examination showed that 

 ovarian tissue was confined to the right ovarian site. There was nothing- 

 left of either graft. 



Guinea-pig No. 1907, black, age three weeks, whose parents were one 

 black , the other cinnamon agouti marked with cream, was grafted with 

 ovaries from a cinnamon agouti, age five days. One graft was dropped 

 into the abdominal cavity, and one was attached to the body-wall on the 

 right side near the incision. Five months later she bore, when mated to male 

 No. 654, fig. 3, a young black male with red brindling. The post-mortem 

 showed at the right body- wall a pinkish mass 1.5 mm. in diameter, which 

 was preserved. Both ovarian sites were also saved for microscopic exam- 

 ination. The peritoneal cavity was negative. The microscope showed 

 that the mass on the body-wall consisted of much fibrous and connective 

 tissue penetrated by blood-vessels, the remains of the degenerated graft. 

 The right ovarian site showed normal ovarian tissue with Graafian follicles 

 present. 



The other seven cases, in which no young were produced, but in which 

 the presence of regenerated ovarian tissue was demonstrated by the micro- 

 scope, need not be given in detail. A well-developed condition of the uterus 

 was observed in all. 



Group IV. 



In this group are included those cases, forty-three in all, which showed, 

 by complete atrophy of the genital tract, absence of all ovarian tissue. Care- 

 ful microscopic inspection was made in all cases, but nothing resembling- 

 ovarian tissue was in any case observed. Four of these animals died from 

 two to nine months after the operation ; the remainder were killed at periods 

 ranging from four months to one year afterward . The average age of the 

 lot at the time of the operation was eight weeks. The individual age of 

 the transplanted ovaries varied from one day to two months, while the 

 average age was three weeks. 



