26 



Influence of Inanition on Metabolism. 



small quantities that are necessarily vaporized from the mouth and neck of 

 the bottle. However, for the purpose of heat measurement it is immaterial 

 in this form of calorimeter whether a gram of water is vaporized in the body 

 of the subject or from the neck of the bottle. In this experiment it is not 

 surely known at what definite times water was drunk, or the actual amount 

 consumed at any given time, but the total amount for each 24 hours is known, 

 and from the above data the periods during which the water was consumed 

 and the amount of water have been estimated. Since, as will be seen later, 

 in computing the total heat production for any given period, the amount of 

 drinking-water consumed during that period is a not unimportant factor, these 

 estimates of this amount were necessary. 



Save on the first day, no water was consumed after the subject retired and 

 the amounts consumed were not far from the same each day, ranging from a 

 maximum of 1360.2 grams on the second day to 1188.0 grams on the last day. 

 The water furnished was that from the city supply and no allowance was made 

 for salts or organic matter. The analyses of the water as furnished by the 

 State Bacteriological Laboratory shows in parts per million 56 of total solids, 

 0.028 free and 0.278 of albuminoid ammonia, and 1.90 of chlorine. The 

 amount of water consumed per period and the total amount per day are given 

 in table 2. 



Table 2. Record of water consumed Metabolism experiment No. 59. 



Ueine. 



In order to show the rate of elimination of nitrogenous material during 

 each 24 hours of the fast, the urine was collected in 3-hour periods from 7 a. m. 

 to 10 p. m. It was deemed inadvisable to awaken the subject to collect the 

 urine in 3-hour periods during the night, as previous experience had shown 

 that the subject found it difficult to go to sleep again. The weight in grams, 

 the specific gravity, the reaction, and the total nitrogen (obtained by the 

 Kjeldahl process) of the urine were determined for the different periods. An 

 aliquot of the urine from each period was taken to make a composite sample 

 for the day and a further aliquot of each day's composite sample was combined 

 to make a 3-day composite. In the daily composite samples, determinations 

 of nitrogen and heat of combustion were made, while in the 3-day composite 



