28 Influence of Inanition on Metabolism. 



the sum of the 18 periods was 40.77 grams. Similarly, the determinations 

 of the heat of combustion on the 3-day composite showed 314 calories, while 

 the determination of the daily composites showed that the urine contained 308 

 calories of energy. In addition to the data in table 3. the total 3-day composite 

 yielded 95.4 per cent of water, 0.87 per cent of carbon, 0.26 per cent of 

 hydrogen in organic matter, and 0.69 per cent of ash. The heat of combustion 

 of the urine per grm was 0.059, 0.108, and 0.119 calorie, respectively, and the 

 total energy of the urine for the 3 clays was 91, 106, and 111 calories, 

 respectively. 



Weight and composition of urine. The total amounts of nitrogen, carbon, 

 hydrogen in organic matter, oxygen, water, solids, ash, and organic matter 

 excreted per day are shown in table 4. The records given in table 3 show that 

 only the determinations of nitrogen and heat of combustion were made daily, 

 the determinations for water, ash, carbon, and hydrogen being made upon the 

 3-day composite. In accordance with our previous custom, the amounts of 

 carbon and hydrogen were apportioned between the 3 days according to the 

 amounts of nitrogen excreted, it being assumed that a definite amount of 

 nitrogen will carry with it a proportionate amount of carbon and hydrogen in 

 organic matter. 



Method of proportioning ash of urine. Since in this experiment the analy- 

 sis of ash was made only on the 3-day composite of the urine, it became neces- 

 sary, in order to obtain the material katabolized from the body for each indi- 

 vidual day, to apportion this ash among the different days of the experiment. 

 The results of subsequent experiments in which the total solids, ash, and 

 nitrogen were determined daily showed that in fasting experiments the ratio 

 of the nitrogen to the total solids is fairly constant, and that on the average 

 N = total solids X 0.29. This percentage was applied to the nitrogen found 

 in experiment No. 59, and the following results for total solids were obtained : 

 For the first day, 40.9 grams; for the second day, 48.4 grams; and for the 

 third day, 50.9 grams a total of 140.2 grams. The actual total solids were 

 determined in the 3-day composite sample and found to be 158.8 grams, a 

 result 18.6 grams greater than the amount computed for the 3 days. For lack 

 of a better method of apportionment, this discrepancy was distributed over the 

 3 days in proportion to the several amounts of solids. The estimated amount 

 of total solids for the first day (40.9 grams) was therefore increased by a 

 certain proportion of the total discrepancy corresponding to the fraction 



~| X (158.8 140.2). Apportioning this error, 18.6 grams, over the 



3 days by the method indicated above, the corrected total solids are 46.3, 54.8, 

 and 57.7 grams. They are recorded in line c of table 4. 



The total solids consist of ash, urea, and other compounds which may be 



