424 Influence of Inanition on Metabolism. 



of two days' duration in which no drinking-water was taken, and the other of 

 four days during which water was consumed. The subject remained in the 

 respiration chamber the larger part of each 24 hours. Usually the periods 

 during which the experimental observations were recorded were of 9 to 10 

 hours' duration, and from the results obtained during these periods, the 

 amounts for 24 hours were computed. On the 2 days of the first experiment 

 the water of respiration and perspiration amounted to 961 and 831 grams, 

 respectively, and for the 4 days of the second experiment, the amounts were 

 730, 709, 597, and 713 grams. Although the subject spent a portion of the 

 day outside the respiration chamber with somewhat greater muscular activity, 

 these results are not liable to the same criticism raised regarding the obser- 

 vations made on J. A., since the loss of body-weight does not enter into the 

 computation for 24 hours. 



The Pashutin respiration apparatus was likewise used in an experiment 

 in which the water of respiration and perspiration was determined by 

 Likhachev. 101 In this experiment the subject remained 26 hours and 10 

 minutes inside the chamber, and from the observations thus made, the amounts 

 for 24 hours were computed, thus marking a distinct advance in accuracy over 

 the experiments of Sadovyen. In the 1-day fasting experiment, the water- 

 vapor output was 664 grams, or 10.68 grams per kilo of body-weight. No 

 records are given of changes in weight of the furniture and other articles inside 

 the chamber. 



In the four earlier fasting experiments made in this laboratory and pre- 

 viously reported, 102 the water of respiration and perspiration was determined. 

 No attempt was made in these experiments to allow for any changes in weight 

 of the bedding and furniture. The amounts of water of respiration and 

 perspiration for the different days were: experiment No. 36 (1 day), 768 

 grams; experiment No. 39 (1 day), 822 grams; experiment No. 42 (1 day), 

 842 grams; experiment No. 51 (2 days), 1018 and 895 grams, respectively. 



The particular type of respiration apparatus used in these experiments is 

 especially adapted for the measurement of the quantity of water in the 

 ventilating air-current. Furthermore, since in all but one (experiment No. 

 59) of the experiments here reported, special precautions were taken to secure 

 reliable weighings of the bedding and other articles in the chamber of the 

 apparatus, corrections for variations in the moisture content of these articles 

 could be applied to the other measurements of water-vapor and the water of 

 respiration and perspiration be accurately measured. Without the use of a 

 respiration chamber and accurate balance for noting the gain or loss in weight 

 of the articles in the chamber, accurate measurements of the water of respira- 

 tion and perspiration are impossible. 



101 The production of heat hy healthy man in a condition of comparative rest. 

 A. A. Likhachev, Inaug. Dissertation (Russian), 1893, St. Petersburg. 



102 U. S. Dept. Agr., Office of Expt. Sta. Bui. 136. 



