30 THE VENOM OF HELODERMA. 



(lc) Gland Removed 35 Minutes after Injection of Pilocarpine. 



The appearance of this gland was very similar to the preceding, the chief 

 difference being that the number of intralobular duct cells containing granules 

 was considerably less and that the granules were fewer in each cell; but in one 

 place near the cut surface of the gland granules were observed in abundance. 

 In the lumina of the tubules a moderate quantity of the granular secretion was 

 present, the amount being slightly more abundant than in 1b. 



(Id) Gland Removed 50 Minutes after Injection of Pilocarpine. 



Granules appeared to be almost entirely absent in the intralobular duct 

 cells, only a few cells in the more distal portions of the tubules showing any 

 clear trace of them. The greater number of these showed the granules in what 

 appears to be an incipient stage of formation, namely, as dark-staining nodular 

 thickenings of the cytoplasmic reticulum. The majority of the granules were 

 minute, but some were larger and closely resembled the typical granules. Fre- 

 quently the granule-containing cells were observed to be much swollen, the 

 adjoining cells, which had not yet begun to reform the granules, being com- 

 pressed into a narrow space. 



A few cells were observed each containing two nuclei, usually in process of 

 disintegration; but such cells were excessively rare, and, owing to the difficulty 

 of finding and of clearly identifying them, it is doubtful if they are normal con- 

 stituents of the gland. They are probably not a result of the pilocarpine 

 stimulation. 



In the lumen of the tubules a granular secretion was present, but less in 

 amount than in the unstimulated gland. At certain places in this secretion 

 were observed many apparently pycnotic cells and nuclei. Whether these 

 were desquamated into the lumen in the normal process of secretion or were 

 merely detached as a result of the pressure to which the gland was subjected in 

 the process of removal from the animal we are unable to solve through a study 

 of the sections. 



Experiment II. 



An animal (C) was injected with 0.1 grain of pilocarpine, a portion of the 

 unstimulated gland having been previously removed and fixed in Kopsch fluid 

 as a control. The animal was then injected and 10 minutes after the injection 

 another portion was removed and fixed. 



(C 1 ) Normal, Unstimulated Gland. 



This gland appeared to be normal, though in many of the tubules the cells, 

 or a considerable number of them, were devoid of granules; but such a condi- 

 tion is not unusual for normal glands, as shown, for example, by the series of 

 sections on which the description of the normal gland was based and in which 

 many of the lobules were mentioned as showing only a few granule-containing 

 cells, while in others the cells were crowded with granules. A similar condi- 

 tion is shown by this gland. In many lobules numerous cells containing the 

 granules were observed, while in others the cells were empty. 



