C. TRANSPLANTATION OF THE VENOM GLAND. 



By Henry Fox and Leo Loeb. 



With one exception all the experiments mentioned under this heading 

 were carried on in the early summer of 1909. Two series of transplantations 

 were planned. In the first series a portion of the gland was cut out and trans- 

 planted to the right side of the thorax of the same animal, where it was inserted 

 within the muscles just underlying the skin. In the second series another por- 

 tion of the same gland was transplanted to a similar place on the left side of a 

 different individual. The transplanted glands were then removed after inter- 

 vals of one, two, three, and four weeks, respectively, fixed, and sectioned. In 

 every case where a transplanted gland was removed, a piece of the normally 

 situated injured gland and one of the uninjured gland were removed and 

 studied as controls. 



Series I. 

 Experiment A. Gland Examined One Week after Transplantation. 



The animal H was operated upon on June 4. A portion of the right gland 

 was cut out and divided into two pieces. One piece, designated H 1 , was trans- 

 planted to the right thorax of the same individual; the other, designated K 2 , 

 was transplanted to the left thorax of another animal, K. 



One week later (June 11) the piece H 1 was removed and fixed in Kopsch 

 fluid. Previous to fixation a small section was cut off and transplanted into 

 an albino mouse, which died a day or two later. Examination of the gland 

 showed the central area completely necrotic, the entire mass being composed 

 of an irregular aggregation of opaque, more or less hyaline particles, among 

 which were scattered numerous, small, densely-staining granules representing 

 necrotic nuclei. Surrounding the necrotic area more or less completely was a 

 middle zone of pyenotic elements. In this zone the individual cells were recog- 

 nizable, but much shrunken; their nuclei were shriveled and closely pressed 

 against the basement-membrane. 



The peripheral portion consisted in part of an extensive zone of nearly 

 normal gland-tissue. In this zone the terminal acini were recognizable, but 

 their typical structure was obscured by a process of desquamation whereby the 

 central lumen had become largely filled with necrotic cells. The living cells of 

 the acini were quite large and apparently normal, except that the cytoplasm 

 was less clearly alveolar in structure and tended to collect in minute droplets, 

 thereby giving the cells a decidedly granular appearance. As a rule, the 

 nuclei were also more shrunken than those of the normal gland-cells. In many 

 cases the chromatin was densely aggregated throughout the entire nucleus; in 



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