GENERAL PROPERTIES AND ACTIONS OF THE VENOM. 



61 



Rat 33, 120 g. 



June 5, 4 h 22 m injected 0.05 c.c. venom intraperitoneally 

 symptoms. 

 Rat 5*4, 190 g. 

 June 7, 5 h 27 m injected intraperitoneally 0.2 c.c. venom. 

 o'clock dead. 

 Rat 56, 120 g. 

 June -, 5 h 28 m injected subcutaneously 0.1 c.c. venom. 

 8 o'clock dead. 

 Un filtered Venom. 



Rat 30, 140 g. 



June 5, 4 h 03 m injected intraperitoneally 0.1 c.c. venom. 

 June 6, 9 a. m., found dead. 

 Rat 32, 120 g. 



June 5, 4 h 01 m injected 0.05 c.c. venom intraperitoneally. 

 symptoms. 

 Rat 55, 190 g. 



June 7, 5 h 25 m injected intraperitoneally 0.2 c.c. venom. 

 o'clock dead. 

 Rat 57, 130 g. 

 June -, 5 h 80 m injected subcutaneously 0.1 c.c. venom. 

 8 o'clock dead. 



June 6, well, na 



G h 30 m dying. 8 



6 h 30 m slightly sick. 



6 h S0 m drowsy. 



June 6, well, no 



6 h 80 m dying. 8 



6 h 80 m slightly sick. 



INFLUENCE OF THE ADDITION OF ACID TO VENOM SOLUTION. 



In connection with certain experiments which we performed it was of 

 interest to determine whether the addition of acid to a solution of venom in- 

 fluenced the toxicity of this substance. It has been shown that the venom, in 

 a solution of either fresh or previously dried venom, is neutral in reaction. We 

 added to 5 c.c. of a 10 per cent (fresh) venom solution 2 c.c. of a N/10 HC1 

 solution. No precipitate was produced. After allowing this mixture to stand 

 for some time we added 2 c.c. of N/10 NaOH solution in order to neutralize 

 the previously added acid. 



Various quantities of this venom solution were injected into mice, and at 

 the same time other mice were injected with similar quantities of the pure, 

 equally diluted venom solution. In every case the mice injected with the neu- 

 tralized acid solution of venom died in approximately the same length of time 

 as the animals injected with the pure venom. The addition of acid to helo- 

 derma venom does not, therefore, change the toxicity of the venom. 



Influence of addition of acid to venom solution. 



EFFECT OF ROENTGEN RAYS ON SECRETION OF VENOM GLAND. 



In order to test the influence of exposure to Roentgen rays on the proper- 

 ties or secretion of the venom we exposed several of the helodermas to the Roent- 

 gen-ray illumination. Finding that single exposures produced no appreciable 

 result, we subjected two helodermas to a period of treatment extending over a 

 period of about 3 weeks. The lower jaws of the animals, with the venom 



