64 THE VENOM OF HELODERMA. 



Rat 14, 98 g. 



May 31, 12 h 10 m injected subcutaneously 0.1 c.c. venom in 1 c.c. NaCl 0.85 per cent. 



12 h 30 m lving on side; breathing rapid. 12 h 40 m lying on side; breathing slow. 



12 h 4o m reflexes present. l h OO m reflexes absent. l h 10 m better; able to move 



about. 2 h 00 m lies on side; apparently dying; reflexes present. 2 h 50 m dead. 



Dog 1, 10 kg. 



May 31, 3 h 14 m given subcutaneously 0.1 c.c. fresh venom. S b 25 m vomited; urine 

 and feces voided. S h 30 m lying down ; very sick. 3 h 50 m vomited. June 

 1, died during forenoon. 

 Dog 2, 9700 g. 



May 31, 3 h 17 m given subcutaneously 0.02 c.c. fresh venom. 3 h 30 m lying down; 

 trembles; occasionally much twitching. 3 h 40 m voids urine and feces. 

 Recovered. 

 Dog 3, 11 kg. 



May 31, 3 h 30 m given 1.1. c.c. fresh venom subcutaneously. 3 h 45 m vomited; urine 

 and feces voided. Very sick all afternoon. Died during night. 

 Cat 1, 3000 g. 



June 1, 12 h 10 m give subcutaneously 0.4 c.c. fresh venom. 12 h 20 m vomits. 12 h 25 m 

 pupils enormously dilated; vomits. 12 h 30 m very nervous; cries if touched; 

 breathing difficult. 2 h 00 m breathing rapid, superficial, pupils still dilated. 

 Sick for some days, but recovered. 

 Cat 2, 3500 g. 



June 1, 12 h 08 m given subcutaneously 0.09 c.c. of fresh venom. 12 h 25 m pupils enor- 

 mously dilated. 12 h 40 m breathing difficult. 2 h 00 m breathing rapid, super- 

 ficial, pupils dilated. Recovered. 

 Cat 3, 3000 g. 



June 1, 12 h 06 m given subcutaneously 0.045 c.c. of fresh venom. 12 h 25 m pupils enor- 

 mously dilated, vomits. 12 h 40 m pupils contracting. Recovered. June 

 24 sick, killed; no lesions. 

 Cat 4, 3000 g. 



June 3, 2 h 12 m given 0.9 c.c. of fresh venom subcutaneously. 2 h 20 m violent move- 

 ments of head from side to side, cries, pupils enormously dilated, eyes roll. 

 2 h 35 m more quiet; eyes still roll, but head movements stopped. 2 h 55 m lies 

 quiet, very nervous; makes crying movements, but has nearly lost voice; breath- 

 ing difficult, audible. 3 h 06 no longer able to hold up head; voice gone. 

 June 4 living; takes no food. June 6 dead. 

 Cat 7, 1500 g. 



June 8, 12 h 55 m injected intraperitoneally 0.6 c.c. of fresh venom. l h 05 m pupils 

 greatly dilated, losing voice. l h 40 m pupils contracted, voice gone; breathing 

 labored; tears secreted. Recovered. 

 Cat 9, 700 g. 



June 8, 2 h 25 m injected intraperitoneally 0.7 c.c. of fresh venom. 2 h 35 m very much 

 affected; pupils dilated; tears and saliva flowing; breathing forced. 5 h 00 m 

 has remained in apparently dying condition all afternoon; breathing forced; 

 nearly paralyzed. 5 h 30 m dead. 



CONDITION OF MOUSE'S EYE PRODUCED BY INJECTION OF 



HELODERMA VENOM. 



We found that the injection of heloderma venom into mice caused fre- 

 quently a peculiar condition of the eye. This condition did not appear as a 

 rule when mice were injected with a large quantity of venom a quantity 

 which was lethal within a comparatively short time but appeared usually 

 when quantities of venom had been injected which caused death after many 

 hours or several days. At times the injection of a sublethal dose of venom was 

 sufficient to produce this lesion. However, its appearance was not constant, 

 even after the injection of a lethal dose. 



The lesion was observed as early as from \\ to 6 hours after the injection 

 of the venom. At this time the eyeball appeared somewhat more prominent 

 than usual and the cornea showed a slight opacity. The protrusion of the eye 

 and opacity of the cornea progressed fairly rapidly (there was, however, con- 



