244 THE VENOM OF HELODERMA. 



No. 20. The same as No. 19, except that it remained in the incubator 12 hours 

 longer. Acidity = 4.7 c.c. N/10 potassium hydroxid or, subtracting 

 control (No. 18), 2.6 c.c. Evidently keeping the medium neutral did 

 not have a very great effect. 



No. 21. 1 c.c. 1 per cent venom solution heated to 100 C. for 30 minutes, 10 

 c.c. 1 per cent lecithin emulsion, 1 c.c. water, 3 drops toluol. After 

 incubation and subtraction of control (No. 16) acidity = 0.2 c.c. 

 N/10 potassium hydroxid. Evidently boiling, as also appears from 

 the first series of experiments, destroys the lipolytic power. 



Dry Venom Heated to 60. 



All the experiments were performed exactly as in the first series, so that 

 they need not be redescribed. The following results were obtained: 



Diastase: Absent. 

 Pepsin: Present. 

 Trypsin: Probably absent. 



Lipase: After 6 hours' digestion, subtracting control, 0.3 c.c. N/10 alkali required. 

 After 12 hours' digestion, 0.8 c.c. N/10 alkali. 



Dried Blood of Heloderma, 1910. 



Diastase: Doubtful. 

 Pepsin: Absent. 



Trypsin: Slight. (Casein method only.) 



Lipase: After 6 hours, subtracting control, 0.3 c.c. N/10 alkali. After 12 hours, 0.5 

 c.c. N/10 alkali. 



Dried Blood of Heloderma (Second Specimen). 



Diastase: Weak. 

 Pepsin: Absent. 



Trypsin: Present, slight. (Casein method only.) 



Lipase: After 6 hours 0.2 c.c. N/10 alkali required. After 12 hours 0.6 c.c. N/10 

 alkali required. 



The Venom Gland (Dried and Powdered). 



Diastase: Absent. 

 Pepsin: Trace. 



Trypsin : Doubtful trace, probably absent. (Casein method only.) 

 Lipase: After 6 hours' incubation required 0.6 c.c. N/10 alkali. After 12 hours' incuba- 

 tion required 1.1 c.c. N/10 alkali. 



The absence of even a trace of diastase in the dried gland is interesting. 

 The diastase present in the venom must be supplied by some one of the other 

 glands pouring their secretion into the mouth. 



