EXPERIMENTAL. 

 Preparations j, 4, and 5. 



21 



Another preparation of albumin was made by extracting with 10 per cent 

 sodium chloride solution 2000 grams of so-called ' ' shorts ' ' from the spring- 

 wheat flour. This substance consisted chiefly of particles of the outer coats 

 of the seed to which more or less of the adjacent embryo and endosperm 

 adhered. After three hours the extract was strained through a coarse cloth 

 and squeezed out from the residue in a screw-press. After the starch had 

 settled, the nearly clear extract, which had a deep red-brown color, was 

 siphoned off and saturated with ammonium sulphate. The precipitate thus 

 produced was filtered off and dissolved in 10 per cent sodium-chloride brine. 

 The resulting solution, filtered from all the insoluble matter, was then 

 dialyzed free from chlorides, the precipitated globulins filtered off, and the 

 albumin contained in the solution separated by heating to 65 . This 

 preparation, 5, had the composition shown in the preceding table. 



Table i. Summary of analyses of coagulated wheat albumin. 



Carbon . . . 

 Hydrogen 

 Nitrogen . 

 Sulphur . . 

 Oxygen. . . 



The agreement of the figures in table 1 is satisfactory, with the exception 

 of the nitrogen in 4. The accuracy of this analysis in this respect, however, 

 can not be doubted, as four determinations of this element were made, all 

 of which agreed closely. As this preparation was separated at a higher 

 temperature than any of the others, it is possible that it had in consequence 

 lost some of its nitrogen. 



