EXPERIMENTAL. 



27 



To separate globulin from the aqueous extract 1200 cc. of clear, filtered 

 extract were obtained from 200 grams of the germ meal treated with 2000 cc. 

 of water. 1000 cc. of this extract were dialyzed in running water for 6 

 days, and the large precipitate resulting gave preparation 22, weighing 9.17 

 grams. 



These preparations, thus variously obtained from the aqueous extract, 

 were dried to constant weight at no and analyzed with the results given 

 in table 2, most of the figures being the average of closely agreeing duplicate 

 determinations. 



Table 2. Composition of preparations of protein from the water extract of 



the wheat embryo. 



Carbon. . . . 

 Hydrogen . 

 Nitrogen . . 

 Sulphur . . . 

 Phosphorus 

 Ash 



P 2 O s in ash 



14. 



P. ct. 



52.28 



6.97 



16.38 



i-39 

 0.07 



0.44 



trace 



Carbon 



Hydrogen . 

 Nitrogen . . . 

 Sulphur . . . 

 Phosphorus 



Ash 



P 2 Oj in ash. 



22. 



P. ct. 



49-59 

 6.68 



16.34 

 0.91 



1.85 

 2.50 

 1.79 



Assuming that those of the foregoing preparations which contain phos- 

 phorus are compounds of protein with the nucleic acid which has been 

 separated from the aqueous extract of wheat germs, 1 and also assuming that 

 all the phosphorus of these preparations is a part of the nucleic acid, the 

 composition of these preparations was calculated free from nucleic acid. 

 The analyses were further calculated ash-free by subtracting the P 2 5 

 contained in the ash from the total ash, which seems permissible, since 

 the ash consisted almost wholly of metaphosphates of potassium and sodium, 



1 Osborne & Campbell, Journal American Chemical Society, 1900, xxii, p. 379 ; also 

 Osborne & Harris, Report Connecticut Agricultural Experiment Station for 1901, p. 365. 



