34 THE PROTEINS OF THE WHEAT KERNEL. 



The aqueous layer was saponified by warming with an excess of barium 

 hydroxide on the water-bath for 7 hours. After standing for some time 

 the crystals of racemic barium aspartate were filtered out and decomposed 

 with an equivalent quantity of sulphuric acid. The filtrate from the barium 

 sulphate gave, on concentration, 5.06 grams of aspartic acid, which, when 

 recrystallized from water, was analyzed. 



Carbon and hydrogen : 0.24S3 gram substance, dried at no , gave 0.3278 gram CO, and 



0.1 2 15 gram H 2 0. 

 Nitrogen : 0.2769 gram substance gave NH 3 = 2.93 cc. HC1 (1 cc. HCl = o.oi gram N). 

 Calculated for C 4 H.0 4 N, C 36.06, H 5.31, N 10.55 P- ct. ; found, C 36.00, H 5.44, N 



10.59 P- ct. 



The filtrate from barium aspartate was freed from barium quantitatively 

 with sulphuric acid and the filtrate from the barium sulphate concentrated to 

 small volume and saturated with hydrochloric acid gas. After long standing 

 on ice, 2.15 grams of glutamiuic acid hydrochloride separated. 



The filtrate from this was evaporated under reduced pressure, the residue 

 taken up in water, and the chlorine removed with silver sulphate. 



After removing the sulphuric acid with an equivalent quantity of barium 

 hydroxide, the solution was boiled with an excess of copper hydroxide. The 

 filtered solution, on standing, separated tyrosine-like needles of copper laevo- 

 aspartate which weighed 7.41 grams, equivalent to 3.57 grams of aspartic 

 acid. 



Nitrogen : 0.1853 gram substance, air-dried, gave NH 3 = o.93 cc. HC1 (1 cc. HC1 = 0.01 



gram N). 

 Copper : 0.1677 gram substance, air-dried, gave 0.0491 gram CuO. 

 Calculated for C 4 H 5 4 N, Cu $% H 2 0, N 5.09, Cu 23.06 p. ct.; found, N5.02, Cu 23.37 



p. ct. 



From the copper salt the free acid was regenerated and analyzed. 



Carbon and hydrogen: 0.2041 gram substance gave 0.2715 gram C0 2 and 0.1054 gram 



H 2 0. 

 Calculated for C 4 H 7 4 N, C 36.06, H 5.31 p. ct.; found, C 36.29, H 5.74 p. ct. 



Specific rotation. Dissolved in 20 per cent hydrochloric acid, 



(a) ^ = +2 3 .8 



Fischer & Dorpinghaus 1 found 



/ n 2 1 O 



()- 5 -= + 22.0 



An effort to isolate serine in the filtrate from the copper aspartate failed. 

 Fischer & Dorpinghaus, Zeitschrift fur physiologische Chemie, 1902, xsxvi, p. 462. 



