3^ THE) PROTEINS OF THE WHEAT KERNEL. 



Arginine, Histidine, and Lysine. 



The filtrate from the tyrosine, by Kossel's method, yielded a solution in 

 which the nitrogen found corresponded to 0.99 gram of histidine, or 2.83 

 per cent. 



The identity of the histidine could not be established owing to its very 

 small amount. 



The solution of the arginine contained nitrogen equal to 0.6720 gram, 

 which is equal to 2.08 grams of arginine, or 5.94 per cent. A part was con- 

 verted into the nitrate and this latter into the copper salt by boiling with an 

 excess of copper hydroxide and the copper salt recrystallized from water. 



Water: 0.19S4 gram substance, air-dried, lost 0.019 1 gram H 2 at no . 

 Copper : 0.1766 gram substance, dried at no , gave 0.0262 gram CuO. 

 Calculated for C 12 H 28 4 N 8 Cu (N0 3 ) 2 ' 3 H 2 0, H 2 9.15 p. ct.; found, H 2 9.62 p. ct. 

 Calculated for C 12 H 28 4 N 8 Cu (N0 3 ) 2 , Cu 11.85 P- ct.; found, Cu 11.83 P- ct. 



The filtrate from the copper salt was freed from copper and the solution 

 evaporated with an excess of sulphuric acid under reduced pressure. The 

 sulphuric acid was removed with an excess of barium hydroxide, and the 

 barium with carbonic acid. The filtrate from the barium carbonate was evap- 

 orated to dryness and the arginine converted into the picrolonate, according 

 to the directions of Steudel. This melted at 226 to 227 ; Steudel gives 225 . 



Nitrogen: 0.0516 gram substance, dried at no , gave 11. 8 cc. moist N 3 at 25 and 



765 mm. 

 Calculated for C 6 H u 2 N 4 C 10 H 8 O 5 N 4 , N 25.62 p. ct. ; found, N 25.71 p. ct. 



The filtrate from the silver precipitate which contained the arginine and 

 histidine was freed from silver and barium, and the lysine precipitated with 

 phosphotungstic acid and then converted into the picrate, of which 2.47 

 grams, equal to 0.9616 gram of lysine, or 2.75 per cent, was obtained. 

 This was recrystallized from water and analyzed. 



Nitrogen: 0.2288 gram substance gave 38.6 cc. moist N 2 at 25. 5 and 759 mm. 

 Calculated for C 6 H u 2 N 2 C 6 H 3 7 N 3 , N 1S.70 p. ct. ; found, N 18.77 p. ct. 



The results of this hydrolysis are given in table 5. 



Table 5. Leucosin. 



p. ct. 



Glycocoll 0.94 



Alanine 4.45 



Aminovalerianic acid 0.18 



Leucine n-34 



a-proline 3. 18 



Phenylalanine 3-83 



Aspartic acid 3.35 



Glutaminic acid 6.73 



P. ct. 



Tyrosine 3.34 



Lysine 2 75 



Histidine 2.83 



Arginine 5.94 



Ammonia 1.41 



Tryptophane present 



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